ArticleJournal of bacteriology2010
P1 plasmid segregation: accurate redistribution by dynamic plasmid pairing and separation.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Overview of Type II and III Toxin-Antitoxin Systems with a Focus on Druggability.Toxins · 2018Pooled it
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- Brownian ratchet mechanisms for carboxysome positioning in bacteria.Current opinion in microbiology · 2025Review
- Reclassification of the first Bacillus tropicus phage calls for reclassification of other Bacillus temperate phages previously designated as plasmids.BMC genomics · 2024Article
- Plasmid partitioning driven by collective migration of ParA between nucleoid lobes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- VirB, a key transcriptional regulator ofmBio · 2023Article
- VirB, a key transcriptional regulator ofbioRxiv : the preprint server for biology · 2023Article
- Distinct architectural requirements for theeLife · 2022Article
- Chemophoresis engine: A general mechanism of ATPase-driven cargo transport.PLoS computational biology · 2022Article
- Dissection of the ATPase active site of McdA reveals the sequential steps essential for carboxysome distribution.Molecular biology of the cell · 2021Article
- Spatial control over near-critical-point operation ensures fidelity of ParABS-mediated DNA partition.Biophysical journal · 2021Article
- VirB, a key transcriptional regulator of virulence plasmid genes inJournal of bacteriology · 2021Article
- Putative plasmid prophages of Bacillus cereus sensu lato may hold the key to undiscovered phage diversity.Scientific reports · 2021Article
- The emergence of phase separation as an organizing principle in bacteria.Biophysical journal · 2021Review
- Liquid-Liquid Phase Separation: Unraveling the Enigma of Biomolecular Condensates in Microbial Cells.Frontiers in microbiology · 2021Review
- Article
- Origin and Evolution of Carboxysome Positioning Systems in Cyanobacteria.Molecular biology and evolution · 2020Article
- Can a Flux-Based Mechanism Explain Protein Cluster Positioning in a Three-Dimensional Cell Geometry?Biophysical journal · 2019Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
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4 authors.
Funding
Abstract
Low-copy-number plasmids, such as P1 and F, encode a type Ia partition system (P1par or Fsop) for active segregation of copies to daughter cells. Typical descriptions show a single central plasmid focus dividing and the products moving to the cell quarter regions, ensuring segregation. However, using improved optical and analytical tools and large cell populations, we show that P1 plasmid foci are very broadly distributed. Moreover, under most growth conditions, more than two foci are frequently present. Each focus contains either one or two plasmid copies. Replication and focus splitting occur at almost any position in the cell. The products then move rapidly apart for approximately 40% of the cell length. They then tend to maintain their relative positions. The segregating foci often pass close to or come to rest close to other foci in the cell. Foci frequently appear to fuse during these encounters. Such events occur several times in each cell and cell generation on average. We argue that foci pair with their neighbors and then actively separate again. The net result is an approximately even distribution of foci along the long cell axis on average. We show mathematically that trans-pairing and active separation could greatly increase the accuracy of segregation and would produce the distributions of foci that we observe. Plasmid pairing and separation may constitute a novel fine-tuning mechanism that takes the basic pattern created when plasmids separate after replication and converts it to a roughly even pattern that greatly improves the fidelity of plasmid segregation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.