ArticleJournal of biomedical optics
Characterization of an orange acceptor fluorescent protein for sensitized spectral fluorescence resonance energy transfer microscopy using a white-light laser.
Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity.Communications biology · 2020Article
- A STING-based biosensor affords broad cyclic dinucleotide detection within single living eukaryotic cells.Nature communications · 2020Article
- MultiplexedContrast media & molecular imaging · 2020Article
- Cyclic-di-GMP regulation promotes survival of a slow-replicating subpopulation of intracellularProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2.Scientific reports · 2017Article
- Direct comparison of shot-to-shot noise performance of all normal dispersion and anomalous dispersion supercontinuum pumped with sub-picosecond pulse fiber-based laser.Scientific reports · 2016Article
- Three-color confocal Förster (or fluorescence) resonance energy transfer microscopy: Quantitative analysis of protein interactions in the nucleation of actin filaments in live cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Multi-color imaging of the bacterial nucleoid and division proteins with blue, orange, and near-infrared fluorescent proteins.Frontiers in microbiology · 2015Article
- Hypoxia-induced changes in protein s-nitrosylation in female mouse brainstem.American journal of respiratory cell and molecular biology · 2015Article
- Progressive accumulation of activated ERK2 within highly stable ORF45-containing nuclear complexes promotes lytic gammaherpesvirus infection.PLoS pathogens · 2014Article
- IQGAP1 interactome analysis by in vitro reconstitution and live cell 3-color FRET microscopy.Cytoskeleton (Hoboken, N.J.) · 2013Article
- Förster resonance energy transfer microscopy and spectroscopy for localizing protein-protein interactions in living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Review
- Single cell optical imaging and spectroscopy.Chemical reviews · 2013Review
- Amyloid-β signals through tau to drive ectopic neuronal cell cycle re-entry in Alzheimer's disease.Journal of cell science · 2013Article
- Red fluorescent proteins: advanced imaging applications and future design.Angewandte Chemie (International ed. in English) · 2012Review
- Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Review
- Three-Color FRET expands the ability to quantify the interactions of several proteins involved in actin filament nucleation.Proceedings of SPIE--the International Society for Optical Engineering · 2012Article
- Article
- Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy.Nature protocols · 2011Article
- FRET microscopy in 2010: the legacy of Theodor Förster on the 100th anniversary of his birth.Chemphyschem : a European journal of chemical physics and physical chemistry · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Orange fluorescent proteins (FPs) are attractive candidates as Forster resonance energy transfer (FRET) partners, bridging the gap between green and red/far-red FPs, but they pose significant challenges using common fixed laser wavelengths. We investigated monomeric Kusabira orange 2 (mKO2) FP as a FRET acceptor for monomeric teal FP (mTFP) as donor on a FRET standard construct using a fixed-distance amino acid linker, expressed in live cells. We quantified the apparent FRET efficiency (E%) of this construct, using sensitized spectral FRET microscopy on the Leica TCS SP5 X imaging system equipped with a white-light laser that allows choosing any excitation wavelength from 470 to 670 nm in 1-nm increments. The E% obtained in sensitized spectral FRET microscopy was then confirmed with fluorescence lifetime measurements. Our results demonstrate that mKO2 and mTFP are good FRET partners given proper imaging setups. mTFP was optimally excited by the Argon 458 laser line, and the 540-nm wavelength excitation for mKO2 was chosen from the white-light laser. The white-light laser generally extends the usage of orange and red/far-red FPs in sensitized FRET microscopy assays by tailoring excitation and emission precisely to the needs of the FRET pair.
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