ArticlePLoS pathogens2009
Functional analysis of the quorum-sensing streptococcal invasion locus (sil).
Article in PLoS pathogens, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Article
- Review
- Overlapping Streptococcus pyogenes and Streptococcus dysgalactiae subspecies equisimilis household transmission and mobile genetic element exchange.Nature communications · 2024Article
- Streptococcal peptides and their roles in host-microbe interactions.Frontiers in cellular and infection microbiology · 2023Review
- The Role of SilX in Bacteriocin Production ofFrontiers in microbiology · 2022Article
- Angicin, a novel bacteriocin of Streptococcus anginosus.Scientific reports · 2021Article
- Alternative approaches to treat bacterial infections: targeting quorum-sensing.Expert review of anti-infective therapy · 2020Review
- Current Understanding of Group A Streptococcal Biofilms.Current drug targets · 2019Review
- Signaling by a Conserved Quorum Sensing Pathway Contributes to GrowthInfection and immunity · 2018Article
- In silico characterisation of the two-component system regulators of Streptococcus pyogenes.PloS one · 2018Article
- Emergence of a Streptococcus dysgalactiae subspecies equisimilis stG62647-lineage associated with severe clinical manifestations.Scientific reports · 2017Article
- Complete Genome Sequence ofGenome announcements · 2017Article
- Identification of a two-component Class IIb bacteriocin in Streptococcus pyogenes by recombinase-based in vivo expression technology.Scientific reports · 2016Article
- TheFrontiers in microbiology · 2016Article
- The role of the local microbial ecosystem in respiratory health and disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Molecular epidemiology and genomics of group A Streptococcus.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2015Review
- RNA-mediated regulation in Gram-positive pathogens: an overview punctuated with examples from the group A Streptococcus.Molecular microbiology · 2014Review
- Molecular epidemiology of sil locus in clinical Streptococcus pyogenes strains.Journal of clinical microbiology · 2014Article
- Peptide pheromone signaling in Streptococcus and Enterococcus.FEMS microbiology reviews · 2014Review
- Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group A streptococcus (GAS) causes a wide variety of human diseases, and at the same time, GAS can also circulate without producing symptoms, similar to its close commensal relative, group G streptococcus (GGS). We previously identified, by transposon-tagged mutagenesis, the streptococcal invasion locus (sil). sil is a quorum-sensing regulated locus which is activated by the autoinducer peptide SilCR through the two-component system SilA-SilB. Here we characterize the DNA promoter region necessary for SilA-mediated activation. This site is composed of two direct repeats of 10 bp, separated by a spacer of 11 bp. Fusion of this site to gfp allowed us to systematically introduce single-base substitutions in the repeats region and to assess the relative contribution of various positions to promoter strength. We then developed an algorithm giving different weights to these positions, and performed a chromosome-wide bioinformatics search which was validated by transcriptome analysis. We identified 13 genes, mostly bacteriocin related, that are directly under the control of SilA. Having developed the ability to quantify SilCR signaling via GFP accumulation prompted us to search for GAS and GGS strains that sense and produce SilCR. While the majority of GAS strains lost sil, all GGS strains examined still possess the locus and approximately 63% are able to respond to exogenously added SilCR. By triggering the autoinduction circle using a minute concentration of synthetic SilCR, we identified GAS and GGS strains that are capable of sensing and naturally producing SilCR, and showed that SilCR can be sensed across these streptococci species. These findings suggest that sil may be involved in colonization and establishment of commensal host-bacterial relationships.
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