ArticlePLoS computational biology2009
Alternative splicing in the differentiation of human embryonic stem cells into cardiac precursors.
Article in PLoS computational biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 93 citations in OpenAlex.
- Article
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- Splicing neoantigen discovery with SNAF reveals shared targets for cancer immunotherapy.Science translational medicine · 2024Article
- Sinus venosus adaptation models prolonged cardiovascular disease and reveals insights into evolutionary transitions of the vertebrate heart.Nature communications · 2023Article
- Broad misappropriation of developmental splicing profile by cancer in multiple organs.Nature communications · 2022Article
- Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate.Nucleic acids research · 2022Article
- QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer.Journal of molecular cell biology · 2021Article
- Prolonged Exposure to Microgravity Reduces Cardiac Contractility and Initiates Remodeling in Drosophila.Cell reports · 2020Article
- Transcriptomic and proteomic signatures of stemness and differentiation in the colon crypt.Communications biology · 2020Article
- A tumor-associated splice-isoform ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Alternative splicing of jnk1a in zebrafish determines first heart field ventricular cardiomyocyte numbers through modulation of hand2 expression.PLoS genetics · 2020Article
- PinX1t, a Novel PinX1 Transcript Variant, Positively Regulates Cardiogenesis of Embryonic Stem Cells.Journal of the American Heart Association · 2020Article
- Proteasome inhibition preserves longitudinal growth of denervated muscle and prevents neonatal neuromuscular contractures.JCI insight · 2019Article
- Maturation of heart valve cell populations during postnatal remodeling.Development (Cambridge, England) · 2019Article
- Article
- Alternative splicing links histone modifications to stem cell fate decision.Genome biology · 2018Article
- miR-196b target screen reveals mechanisms maintaining leukemia stemness with therapeutic potential.The Journal of experimental medicine · 2018Article
- The Genetic Landscape of Hypoplastic Left Heart Syndrome.Pediatric cardiology · 2018Article
- The Role of scaRNAs in Adjusting Alternative mRNA Splicing in Heart Development.Journal of cardiovascular development and disease · 2018Review
- Long non-coding RNAs involved in the regulatory network during porcine pre-implantation embryonic development and iPSC induction.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 1 country.
Funding
Abstract
The role of alternative splicing in self-renewal, pluripotency and tissue lineage specification of human embryonic stem cells (hESCs) is largely unknown. To better define these regulatory cues, we modified the H9 hESC line to allow selection of pluripotent hESCs by neomycin resistance and cardiac progenitors by puromycin resistance. Exon-level microarray expression data from undifferentiated hESCs and cardiac and neural precursors were used to identify splice isoforms with cardiac-restricted or common cardiac/neural differentiation expression patterns. Splice events for these groups corresponded to the pathways of cytoskeletal remodeling, RNA splicing, muscle specification, and cell cycle checkpoint control as well as genes with serine/threonine kinase and helicase activity. Using a new program named AltAnalyze (http://www.AltAnalyze.org), we identified novel changes in protein domain and microRNA binding site architecture that were predicted to affect protein function and expression. These included an enrichment of splice isoforms that oppose cell-cycle arrest in hESCs and that promote calcium signaling and cardiac development in cardiac precursors. By combining genome-wide predictions of alternative splicing with new functional annotations, our data suggest potential mechanisms that may influence lineage commitment and hESC maintenance at the level of specific splice isoforms and microRNA regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.