ArticleThe EMBO journal2010
Strain through the neck linker ensures processive runs: a DNA-kinesin hybrid nanomachine study.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
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- Size-Selective Capturing of Exosomes Using DNA Tripods.Journal of the American Chemical Society · 2024Article
- Evaluating the effect of two-dimensional molecular layout on DNA origami-based transporters.Nanoscale advances · 2023Article
- Information flow, gating, and energetics in dimeric molecular motors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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- Revisiting the Glass Treatment for Single-Molecule Analysis of ncRNA Function.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Synthetic biology approaches to dissecting linear motor protein function: towards the design and synthesis of artificial autonomous protein walkers.Biophysical reviews · 2020Review
- Construction of integrated gene logic-chip.Nature nanotechnology · 2018Article
- Controllable molecular motors engineered from myosin and RNA.Nature nanotechnology · 2018Article
- Parsing the roles of neck-linker docking and tethered head diffusion in the stepping dynamics of kinesin.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Biochemical and single-molecule analyses of the RNA silencing suppressing activity of CrPV-1A.Nucleic acids research · 2017Article
- Structural Correlation of the Neck Coil with the Coiled-coil (CC1)-Forkhead-associated (FHA) Tandem for Active Kinesin-3 KIF13A.The Journal of biological chemistry · 2016Article
- Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.Biophysical reviews · 2015Article
- Article
- The family-specific K-loop influences the microtubule on-rate but not the superprocessivity of kinesin-3 motors.Molecular biology of the cell · 2014Article
- Single-molecule fluorescence and in vivo optical traps: how multiple dyneins and kinesins interact.Chemical reviews · 2014Review
- Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motors.Developmental cell · 2012Review
- Engineered, harnessed, and hijacked: synthetic uses for cytoskeletal systems.Trends in cell biology · 2012Review
- Dynein achieves processive motion using both stochastic and coordinated stepping.Nature structural & molecular biology · 2012Article
- Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The motor protein kinesin has two heads and walks along microtubules processively using energy derived from ATP. However, how kinesin heads are coordinated to generate processive movement remains elusive. Here we created a hybrid nanomachine (DNA-kinesin) using DNA as the skeletal structure and kinesin as the functional module. Single molecule imaging of DNA-kinesin hybrid allowed us to evaluate the effects of both connect position of the heads (N, C-terminal or Mid position) and sub-nanometer changes in the distance between the two heads on motility. Our results show that although the native structure of kinesin is not essential for processive movement, it is the most efficient. Furthermore, forward bias by the power stroke of the neck linker, a 13-amino-acid chain positioned at the C-terminus of the head, and internal strain applied to the rear of the head through the neck linker are crucial for the processive movement. Results also show that the internal strain coordinates both heads to prevent simultaneous detachment from the microtubules. Thus, the inter-head coordination through the neck linker facilitates long-distance walking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.