ArticleJournal of applied physiology (Bethesda, Md. : 1985)2010
Inhibition of calpain prevents muscle weakness and disruption of sarcomere structure during hindlimb suspension.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Gene Manipulation of Muscle Phenotype.Advances in experimental medicine and biology · 2025Review
- Skeletal muscle atrophy, regeneration, and dysfunction in heart failure: Impact of exercise training.Journal of sport and health science · 2023Review
- Article
- Locomotor and respiratory muscle abnormalities in HFrEF and HFpEF.Frontiers in cardiovascular medicine · 2023Review
- Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle.Pflugers Archiv : European journal of physiology · 2022Article
- Review
- The inflammatory response, a mixed blessing for muscle homeostasis and plasticity.Frontiers in physiology · 2022Review
- Plantar mechanical stimulation attenuates protein synthesis decline in disused skeletal muscle via modulation of nitric oxide level.Scientific reports · 2021Article
- Emerging Strategies Targeting Catabolic Muscle Stress Relief.International journal of molecular sciences · 2020Review
- Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?Doklady. Biochemistry and biophysics · 2018Article
- The role of calpains in ventilator-induced diaphragm atrophy.Intensive care medicine experimental · 2017Article
- Reduced skeletal muscle fiber size following caloric restriction is associated with calpain-mediated proteolysis and attenuation of IGF-1 signaling.American journal of physiology. Regulatory, integrative and comparative physiology · 2017Article
- Cessation of biomechanical stretch model of C2C12 cells models myocyte atrophy and anaplerotic changes in metabolism using non-targeted metabolomics analysis.The international journal of biochemistry & cell biology · 2016Article
- Calpain inhibition rescues troponin T3 fragmentation, increases Cav1.1, and enhances skeletal muscle force in aging sedentary mice.Aging cell · 2016Article
- Calcium-dependent phospholipase A2 modulates infection-induced diaphragm dysfunction.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Muscle fibers are injured at the time of acute and chronic rotator cuff repair.Clinical orthopaedics and related research · 2015Article
- Muscle-specific calpastatin overexpression prevents diaphragm weakness in cecal ligation puncture-induced sepsis.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- Disuse-induced muscle wasting.The international journal of biochemistry & cell biology · 2013Review
- Calpain inhibition attenuated morphological and molecular changes in skeletal muscle of experimental allergic encephalomyelitis rats.Journal of neuroscience research · 2012Article
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Authors and funding
3 authors.
Funding
Abstract
Unloading skeletal muscle results in atrophy and weakness. Inhibition of calpain activity during unloading reduced atrophy, but the impact on force generation has not been determined. Our hypothesis was that inhibition of calpain, through muscle-specific overexpression of calpastatin, would prevent the disruption of sarcomere structure and decreased specific force (kN/m(2)) observed during unloading. Calpastatin-overexpressing (cp) and wild-type (wt) mice were subjected to 3, 9, or 14 days of hindlimb suspension (HS). Compared with soleus muscles of non-suspended control mice, soleus muscles of wt mice showed a 25% decline in mass after 14 days of HS while maximum isometric force (P(o)) decreased by 40%, resulting in a specific P(o) that was 35% lower than control values. Over the same time period, muscles of cp mice demonstrated 25% declines in both mass and P(o) but no change in specific P(o). Consistent with the preservation of specific force during HS, soleus muscles of cp mice also maintained a high degree of order in sarcomere structure, in contrast to wt muscles that demonstrated misalignment of z-lines and decreased uniformity of thick filament lengths. Susceptibility to lengthening contraction-induced injury increased with the duration of HS and was not different for muscles of cp and wt mice. We conclude that inhibition of calpain activity during unloading preserves sarcomere structure such that the isometric force-generating capability is not diminished, while the effects of unloading on lengthening contraction-induced injury likely occur through calpain-independent mechanisms.
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