ArticleProceedings of the National Academy of Sciences of the United States of America2009
Rescue and characterization of episomally replicating DNA from the moss Physcomitrella.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Production of human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors.Plant cell reports · 2025Article
- Plastid engineering using episomal DNA.Plant cell reports · 2023Review
- Process Engineering of Biopharmaceutical Production in Moss BioreactorsFrontiers in bioengineering and biotechnology · 2022Article
- A blueprint for gene function analysis through Base Editing in the model plant Physcomitrium (Physcomitrella) patens.The New phytologist · 2021Article
- Mutations in themicroPublication biology · 2021Article
- Stable Protein Sialylation in Physcomitrella.Frontiers in plant science · 2020Article
- Article
- The XPF-ERCC1 Complex Is Essential for Genome Stability and Is Involved in the Mechanism of Gene Targeting inFrontiers in plant science · 2019Article
- Physcomitrella patens, a versatile synthetic biology chassis.Plant cell reports · 2018Review
- Testing of Auxotrophic Selection Markers for Use in the MossFrontiers in plant science · 2017Article
- Simple and Efficient Targeting of Multiple Genes Through CRISPR-Cas9 inG3 (Bethesda, Md.) · 2016Article
- In vivo assembly of DNA-fragments in the moss, Physcomitrella patens.Scientific reports · 2016Article
- RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella.Nucleic acids research · 2014Article
- Physcomitrella patens mutants affected on heat dissipation clarify the evolution of photoprotection mechanisms upon land colonization.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The moss Physcomitrella is unique among plants in that it permits efficient gene targeting by homologous recombination. Furthermore, transformed DNA can replicate episomally in Physcomitrella. Here we show that episomally replicating DNA can be rescued back into Escherichia coli, and we use such rescue to study the fate of the transformed DNA. Significantly, plasmids rescued from moss transformed with circular DNA are identical to the original plasmid, whereas plasmids rescued from moss transformed with linearized DNA frequently have deletions created by direct repeat recombination. These events are highly predictable in that they target the longest direct repeat on the plasmid if this repeat is at least 12 bp. Episomal transformants obtained with linearized DNA show a more than 1,000-fold amplification of the DNA whereas transformants obtained with circular DNA have much lower copy numbers. Most episomal transformants quickly lose the plasmid in the absence of selection, but a semistable type of transformant that loses the plasmid at a much lower frequency was also observed. The consistent rescue of the original plasmid, or of predictable derivatives thereof, suggests that molecular genetics methods which rely on shuttle plasmids are feasible in Physcomitrella.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.