ReviewMethods in enzymology2009
Cell-free translation of integral membrane proteins into unilamelar liposomes.
Review in Methods in enzymology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- SLC45A4 encodes a peroxisomal putrescine transporter that promotes GABA de novo synthesis.Nature communications · 2025Article
- Article
- Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase iniScience · 2024Article
- The biophysical properties of TRIC-A and TRIC-B and their interactions with RyR2.The Journal of general physiology · 2023Article
- Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology.Frontiers in molecular biosciences · 2021Review
- A bacterial biosynthetic pathway for methylated furan fatty acids.The Journal of biological chemistry · 2020Article
- Genomics-enabled analysis of specialized metabolism in bioenergy crops: current progress and challenges.Synthetic biology (Oxford, England) · 2020Review
- A heterodimeric glutathioneThe Journal of biological chemistry · 2019Article
- A forward genetic screen identifies chaperone CNX-1 as a conserved biogenesis regulator ofThe Journal of general physiology · 2018Article
- Switching head group selectivity in mammalian sphingolipid biosynthesis by active-site-engineering of sphingomyelin synthases.Journal of lipid research · 2017Article
- From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins.Structure (London, England : 1993) · 2016Article
- Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein.Protein expression and purification · 2016Article
- Switching head group selectivity in mammalian sphingolipid biosynthesis by active-site engineering of sphingomyelin synthases.Journal of lipid research · 2016Article
- Apoglobin Stability Is the Major Factor Governing both Cell-free and in Vivo Expression of Holomyoglobin.The Journal of biological chemistry · 2015Article
- Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay.Scientific reports · 2015Article
- Mechanisms of integral membrane protein insertion and folding.Journal of molecular biology · 2015Review
- Direct injection of cell-free Kir1.1 protein into Xenopus oocytes replicates single-channel currents derived from Kir1.1 mRNA.Channels (Austin, Tex.) · 2015Article
- DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research.Nucleic acids research · 2014Article
- Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the Pseudomonas aeruginosa prepilin peptidase PilD.MicrobiologyOpen · 2013Article
- Cell-free protein expression systems in microdroplets: Stabilization of interdroplet bilayers.Biomicrofluidics · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Wheat germ cell-free translation is shown to be an effective method to produce integral membrane proteins in the presence of unilamelar liposomes. In this chapter, we describe the expression vectors, preparation of mRNA, two types of cell-free translation reactions performed in the presence of liposomes, a simple and highly efficient purification of intact proteoliposomes using density gradient ultracentrifugation, and some of the types of characterization studies that are facilitated by this facile preparative approach. The in vitro transfer of newly translated, membrane proteins into liposomes compatible with direct measurements of their catalytic function is contrasted with existing approaches to extract membrane proteins from biological membranes using detergents and subsequently transfer them back to liposomes for functional studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.