ReviewMethods in enzymology2009
Purification of recombinant G-protein-coupled receptors.
Review in Methods in enzymology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- To generate functional anti-protease-activated receptor-4 (PAR4), a G protein-coupled receptor, antibodies through PAR4-mRNA-LNP immunization.NPJ vaccines · 2025Article
- Unravelling the mechanism of neurotensin recognition by neurotensin receptor 1.Nature communications · 2023Article
- A robust antibody discovery platform for difficult-to-express G protein-coupled receptors.Protein science : a publication of the Protein Society · 2023Article
- Why Taste Is Pharmacology.Handbook of experimental pharmacology · 2022Article
- Lipid nanoparticle technologies for the study of G protein-coupled receptors in lipid environments.Biophysical reviews · 2020Review
- Combining Neurobehavioral Analysis andACS chemical neuroscience · 2020Article
- The human brain somatostatin interactome: SST binds selectively to P-type family ATPases.PloS one · 2019Article
- Ligand-Induced Coupling between Oligomers of the MBiophysical journal · 2018Article
- Understanding GPCR Recognition and Folding from NMR Studies of Fragments.RSC advances · 2018Article
- Review
- In Situ Reconstitution of the Adenosine AJournal of the American Chemical Society · 2017Article
- Production of Chemokine/Chemokine Receptor Complexes for Structural Biophysical Studies.Methods in enzymology · 2016Article
- Capture-stabilize approach for membrane protein SPR assays.Scientific reports · 2014Article
- Expression, purification, and structural insights for the human uric acid transporter, GLUT9, using the Xenopus laevis oocytes system.PloS one · 2014Article
- Calcium-dependent ligand binding and G-protein signaling of family B GPCR parathyroid hormone 1 receptor purified in nanodiscs.ACS chemical biology · 2013Article
- Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display.Methods (San Diego, Calif.) · 2013Review
- Stabilization of functional recombinant cannabinoid receptor CB(2) in detergent micelles and lipid bilayers.PloS one · 2012Article
- The significance of G protein-coupled receptor crystallography for drug discovery.Pharmacological reviews · 2011Review
- Mass spectrometry-based proteomics of human cannabinoid receptor 2: covalent cysteine 6.47(257)-ligand interaction affording megagonist receptor activation.Journal of proteome research · 2011Article
- Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environment.Journal of biomolecular NMR · 2011Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Structural and functional analysis of most G-protein-coupled receptors (GPCRs) requires their expression and purification in functional form. The produced amount of recombinant membrane-inserted receptors depends on the optimal combination of a particular GPCR and production host; optimization of expression is still a matter of trial-and-error. Prior to purification, receptors must be extracted from the membranes by use of detergent(s). The choice of an appropriate detergent for solubilization and purification is crucial to maintain receptors in their functional state. The initial enrichment can be carried out by affinity chromatography using a general affinity tag (e.g., poly-histidine tag). If the first purification step does not yield pure receptor protein, purification to homogeneity can often be achieved by use of a subsequent receptor-specific ligand column. If suitable immobilized ligands are not available, size exclusion chromatography or other techniques need to be applied. Many GPCRs become unstable upon detergent extraction from lipid membranes, and measures for stabilization are discussed. As an example, the purification of a functional neurotensin receptor to homogeneity in milligram quantities is given below.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.