ArticleJournal of clinical microbiology2010
Oxidative stress of Burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Multi-metal contamination shapes abundance, co-occurrence, and mobility potential of resistance and virulence genes in mining-impacted soils.Infectious medicine · 2026Article
- Laboratory strategies for the identification of Burkholderia species: From classical phenotyping to advanced genomic and proteomic approaches.Antonie van Leeuwenhoek · 2026Review
- Targeted IS-element sequencing uncovers transposition dynamics during selective pressure in enterococci.PLoS pathogens · 2023Article
- Genomic features, antimicrobial susceptibility, and epidemiological insights intoFrontiers in cellular and infection microbiology · 2023Article
- Ecophysiological and genomic analyses of a representative isolate of highly abundant Bacillus cereus strains in contaminated subsurface sediments.Environmental microbiology · 2022Article
- Active Transposition of Insertion Sequences in Prokaryotes: Insights from the Response ofAntioxidants (Basel, Switzerland) · 2022Review
- Frequency, composition and mobility of Escherichia coli-derived transposable elements in holdings of plasmid repositories.Microbial biotechnology · 2022Article
- Mapping the Efficacy and Mode of Action of Ethylzingerone [4-(3-Ethoxy-4-Hydroxyphenyl) Butan-2-One] as an Active Agent againstApplied and environmental microbiology · 2020Article
- What matters in chronic Burkholderia cenocepacia infection in cystic fibrosis: Insights from comparative genomics.PLoS pathogens · 2017Article
- Analysis of Serial Isolates ofAntimicrobial agents and chemotherapy · 2017Article
- Insertion sequences enrichment in extreme Red sea brine pool vent.Extremophiles : life under extreme conditions · 2017Article
- Virulence traits associated withAntimicrobial resistance and infection control · 2017Article
- Review
- Zinc-Induced Transposition of Insertion Sequence Elements Contributes to Increased Adaptability of Cupriavidus metallidurans.Frontiers in microbiology · 2016Article
- Oxidative stress in cystic fibrosis patients with Burkholderia cenocepacia airway colonization: relation of 8-isoprostane concentration in exhaled breath condensate to lung function decline.Folia microbiologica · 2014Article
- Article
- Article
- Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis.BMC genomics · 2011Article
- Insertion sequence-driven evolution of Escherichia coli in chemostats.Journal of molecular evolution · 2011Article
- Identification of cold-temperature-regulated genes in Flavobacterium psychrophilum.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the Czech Republic experienced an epidemic outbreak caused by a B. cenocepacia ST-32 strain. The clonality of the isolates was evident by multilocus sequence typing; however, fingerprinting profiles obtained by pulsed-field gel electrophoresis (PFGE) showed substantial band variability. We investigated whether the PFGE pattern diversity resulted from genomic rearrangements mediated by insertion sequences (IS); in addition, we determined whether stressful growth conditions altered the transposition activity of these IS. DNA probes for IS commonly found in B. cenocepacia were designed using the B. cenocepacia J2315 genome. Southern hybridization analysis of ST-32 isolates demonstrated diversity in both the copy number and the insertion site for a homologue of ISBcen20. Movement of the ISBcen20 homologue was detected when the ST-32 isolate CZ1238 was exposed to oxidative stress (growth in the presence of H(2)O(2)). PFGE analysis of CZ1238 derivatives exposed to oxidative stress demonstrated genomic rearrangements. Interestingly, when the closely related B. cenocepacia strain J2315 was exposed to oxidative stress, no movement of ISBcen20 was detected. Since frameshift mutations are present within the transposases of all copies of this IS in J2315, our data suggest that the transposase is inactive. In summary, we have demonstrated for the first time that IS movement can be mediated by oxidative stress and can lead to genomic rearrangements in the CF pathogen B. cenocepacia. These IS movements may alter the PFGE fingerprints of isolates that are clonal by other typing methods.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.