ArticleJournal of clinical microbiology2010
Diversity of Staphylococcus species strains based on partial kat (catalase) gene sequences and design of a PCR-restriction fragment length polymorphism assay for identification and differentiation of coagulase-positive species (S. aureus, S. delphini, S. hyicus, S. intermedius, S. pseudintermedius, and S. schleiferi subsp. coagulans).
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Comparison of three amplicon sequencing approaches to determine staphylococcal populations on human skin.BMC microbiology · 2021Article
- Virulence factors in coagulase-positive staphylococci of veterinary interest other thanThe veterinary quarterly · 2020Review
- Antimicrobial Resistance of Coagulase-PositiveAntibiotics (Basel, Switzerland) · 2020Article
- Performance of Five Commercial Identification Platforms for Identification of Staphylococcus delphini.Journal of clinical microbiology · 2019Article
- Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland.International journal of environmental research and public health · 2018Article
- High Occurrence ofJournal of environmental and public health · 2018Article
- Evolution of the Population Structure ofFrontiers in microbiology · 2018Article
- Insight into the Genome of Staphylococcus xylosus, a Ubiquitous Species Well Adapted to Meat Products.Microorganisms · 2017Review
- Molecular Epidemiology of Methicillin-ResistantFrontiers in microbiology · 2017Article
- Are Staphylococcus intermedius Infections in Humans Cases of Mistaken Identity? A Case Series and Literature Review.Open forum infectious diseases · 2015Article
- Aerobic Bacterial Community of American Cockroach Periplaneta americana,a Step toward Finding Suitable Paratransgenesis Candidates.Journal of arthropod-borne diseases · 2015Article
- Transcriptomic analysis of Staphylococcus xylosus in the presence of nitrate and nitrite in meat reveals its response to nitrosative stress.Frontiers in microbiology · 2014Article
- Investigations on the efficacy of routinely used phenotypic methods compared to genotypic approaches for the identification of staphylococcal species isolated from companion animals in Irish veterinary hospitals.Irish veterinary journal · 2013Article
- New perspectives for natural antimicrobial peptides: application as antinflammatory drugs in a murine model.BMC immunology · 2012Article
- Production of 2-aminophenoxazin-3-one by Staphylococcus aureus causes false-positive results in β-galactosidase assays.Journal of clinical microbiology · 2012Article
- Staphylococcal response to oxidative stress.Frontiers in cellular and infection microbiology · 2012Review
- Comparative genomics of the Staphylococcus intermedius group of animal pathogens.Frontiers in cellular and infection microbiology · 2012Article
- Complete genome sequence of the veterinary pathogen Staphylococcus pseudintermedius strain HKU10-03, isolated in a case of canine pyoderma.Journal of bacteriology · 2011Article
- Multiplex-PCR method for species identification of coagulase-positive staphylococci.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of degenerate PCR primers was designed and used to amplify and sequence about 75% of the catalase (kat) gene from each of 49 staphylococcal strains. In some strains of Staphylococcus xylosus, S. saprophyticus, and S. equorum, two catalase genes, katA and katB, were found. A phylogenetic tree was generated and showed diversities among 66 partial (about 900-bp) staphylococcal kat nucleotide sequences (including 17 sequences found in GenBank) representing 26 different species. The topology of this tree showed a distribution of staphylococcal species similar, but not identical, to those reported previously based on 16S rRNA, hsp60, sodA, rpoB, tuf, and gap genes. The kat gene sequences were less conserved than those of 16S rRNA, rpoB, hsp60, and tuf genes and slightly more conserved than those of the gap gene. Therefore, kat gene sequence analysis may provide an additional marker for inferring phylogenetic relationships of staphylococci. Moreover, the discrete nucleotide polymorphism revealed in this gene could be exploited for rapid, low-cost identification of staphylococcal species through PCR-restriction fragment length polymorphism (RFLP) analysis. In this study, a PCR-RFLP assay performed by using only the TaqI restriction enzyme was successfully developed for rapid unequivocal identification/differentiation, at species and subspecies levels, of coagulase-positive staphylococci (CPS). The assay was validated by testing the DNA from 100 staphylococcal strains, including reference and wild CPS strains isolated from different environments. This reliable, rapid, and low-cost approach (requiring about 6 h from DNA isolation to the achievement of results and <5 Euros for each strain tested) allowed unambiguous identification of all the strains assayed, including the newly described S. delphini and S. pseudintermedius CPS species.
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