ArticlePlant physiology2010
The nuclear interactor PYL8/RCAR3 of Fagus sylvatica FsPP2C1 is a positive regulator of abscisic acid signaling in seeds and stress.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
- Maize ZmbZIP92 transcription factor positively regulates drought tolerance inPlant signaling & behavior · 2026Article
- Article
- Pan-Genome-Based Characterization of the PYL Transcription Factor Family inPlants (Basel, Switzerland) · 2025Article
- Wheat TaPYL9-involved signalling pathway impacts plant drought response through regulating distinct osmotic stress-associated physiological indices.Plant biotechnology journal · 2025Article
- Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress inInternational journal of molecular sciences · 2025Article
- Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile.Cell reports · 2024Article
- Article
- Time-Series Transcriptomic Analysis of Contrasting Rice Materials under Heat Stress Reveals a Faster Response in the Tolerant Cultivar.International journal of molecular sciences · 2023Article
- Wheat ABA Receptor TaPYL5 Constitutes a Signaling Module with Its Downstream Partners TaPP2C53/TaSnRK2.1/TaABI1 to Modulate Plant Drought Response.International journal of molecular sciences · 2023Article
- Article
- Overexpression VaPYL9 improves cold tolerance in tomato by regulating key genes in hormone signaling and antioxidant enzyme.BMC plant biology · 2022Article
- Genome-wide identification and comparative analysis of the PYL gene family in eight Rosaceae species and expression analysis of seeds germination in pear.BMC genomics · 2022Article
- Mechanisms of Abscisic Acid-Mediated Drought Stress Responses in Plants.International journal of molecular sciences · 2022Review
- Genome-wide identification of ABA receptor PYL/RCAR gene family and their response to cold stress inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Redox feedback regulation of ANAC089 signaling alters seed germination and stress response.Cell reports · 2021Article
- Transcriptional Regulation of Protein Phosphatase 2C Genes to Modulate Abscisic Acid Signaling.International journal of molecular sciences · 2020Review
- Genome-Wide Identification of the ABA Receptors Genes and Their Response to Abiotic Stress in Apple.Plants (Basel, Switzerland) · 2020Article
- Seed comparative genomics in three coffee species identify desiccation tolerance mechanisms in intermediate seeds.Journal of experimental botany · 2020Article
- Analysis of differential gene expression in cold-tolerant vs. cold-sensitive varieties of snap bean (Phaseolus vulgaris L.) in response to low temperature stress.Genes & genomics · 2019Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functional protein phosphatase type 2C from beechnut (Fagus sylvatica; FsPP2C1) was a negative regulator of abscisic acid (ABA) signaling in seeds. In this report, to get deeper insight on FsPP2C1 function, we aim to identify PP2C-interacting partners. Two closely related members (PYL8/RCAR3 and PYL7/RCAR2) of the Arabidopsis (Arabidopsis thaliana) BetV I family were shown to bind FsPP2C1 in a yeast two-hybrid screening and in an ABA-independent manner. By transient expression of FsPP2C1 and PYL8/RCAR3 in epidermal onion (Allium cepa) cells and agroinfiltration in tobacco (Nicotiana benthamiana) as green fluorescent protein fusion proteins, we obtained evidence supporting the subcellular localization of both proteins mainly in the nucleus and in both the cytosol and the nucleus, respectively. The in planta interaction of both proteins in tobacco cells by bimolecular fluorescence complementation assays resulted in a specific nuclear colocalization of this interaction. Constitutive overexpression of PYL8/RCAR3 confers ABA hypersensitivity in Arabidopsis seeds and, consequently, an enhanced degree of seed dormancy. Additionally, transgenic 35S:PYL8/RCAR3 plants are unable to germinate under low concentrations of mannitol, NaCl, or paclobutrazol, which are not inhibiting conditions to the wild type. In vegetative tissues, Arabidopsis PYL8/RCAR3 transgenic plants show ABA-resistant drought response and a strong inhibition of early root growth. These phenotypes are strengthened at the molecular level with the enhanced induction of several ABA response genes. Both seed and vegetative phenotypes of Arabidopsis 35S:PYL8/RCAR3 plants are opposite those of 35S:FsPP2C1 plants. Finally, double transgenic plants confirm the role of PYL8/RCAR3 by antagonizing FsPP2C1 function and demonstrating that PYL8/RCAR3 positively regulates ABA signaling during germination and abiotic stress responses.
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