ArticleMolecular biology of the cell2010
Analyzing the effects of delaying aster separation on furrow formation during cytokinesis in the Caenorhabditis elegans embryo.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Peroxisomal DBP deficiency causes male infertility through disruption of lipid homeostasis in Drosophila.Cellular and molecular life sciences : CMLS · 2026Article
- F-actin organization excludes E-cadherin from the division furrow to ensure cytokinesis fidelity.Life science alliance · 2026Article
- A cortical pool of LIN-5 (NuMA) controls cytokinetic furrow formation and cytokinesis completion.The Journal of cell biology · 2025Article
- Unveiling inter-embryo variability in spindle length over time: Towards quantitative phenotype analysis.PLoS computational biology · 2024Article
- Mechanical and biochemical feedback combine to generate complex contractile oscillations in cytokinesis.Current biology : CB · 2024Article
- Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion.Cell reports · 2023Article
- Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation.eLife · 2022Article
- Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA.Open biology · 2022Article
- Functional midbody assembly in the absence of a central spindle.The Journal of cell biology · 2022Article
- Diverse mechanisms regulate contractile ring assembly for cytokinesis in the two-cell Caenorhabditis elegans embryo.Journal of cell science · 2022Article
- Cortical excitability and cell division.Current biology : CB · 2021Article
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Complementary functions for the Ran gradient during division.Small GTPases · 2021Review
- Importin binding mediates the intramolecular regulation of anillin during cytokinesis.Molecular biology of the cell · 2020Article
- Mitotic Cell Division inGenetics · 2019Review
- Microtubule Feedback and LET-99-Dependent Control of Pulling Forces Ensure Robust Spindle Position.Biophysical journal · 2018Article
- Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity.eLife · 2018Article
- TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis.The Journal of cell biology · 2018Article
- Active Ran regulates anillin function during cytokinesis.Molecular biology of the cell · 2017Article
- LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring.Molecular biology of the cell · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Signaling by the centrosomal asters and spindle midzone coordinately directs formation of the cytokinetic furrow. Here, we explore the contribution of the asters by analyzing the consequences of altering interaster distance during the first cytokinesis of the Caenorhabditis elegans embryo. Delaying aster separation, by using TPXL-1 depletion to shorten the metaphase spindle, leads to a corresponding delay in furrow formation, but results in a single furrow that ingresses at a normal rate. Preventing aster separation, by simultaneously inhibiting TPXL-1 and Galpha signaling-based cortical forces pulling on the asters, delays furrow formation and leads to the formation of multiple furrows that ingress toward the midzone. Disrupting midzone-based signaling, by depleting conserved midzone complexes, results in a converse phenotype: neither the timing nor the number of furrows is affected, but the rate of furrow ingression is decreased threefold. Simultaneously delaying aster separation and disrupting midzone-based signaling leads to complete failure of furrow formation. Based on these results, we propose that signaling by the separated asters executes two critical functions: 1) it couples furrow formation to anaphase onset by concentrating contractile ring proteins on the equatorial cortex in a midzone-independent manner and 2) it subsequently refines spindle midzone-based signaling to restrict furrowing to a single site.
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