ArticleMolecular biology of the cell2010
Sec3-containing exocyst complex is required for desmosome assembly in mammalian epithelial cells.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Regulation of yeast polarized exocytosis by phosphoinositide lipids.Cellular and molecular life sciences : CMLS · 2024Review
- Structure and interactions of the endogenous human Commander complex.Nature structural & molecular biology · 2024Article
- The exocyst complex is an essential component of the mammalian constitutive secretory pathway.The Journal of cell biology · 2023Article
- Desmoglein-2 as a cancer modulator: friend or foe?Frontiers in oncology · 2023Review
- The role of Exo70 in exocytosis and beyond.Small GTPases · 2019Review
- Regulation of Cell Polarity by Exocyst-Mediated Trafficking.Cold Spring Harbor perspectives in biology · 2018Review
- Probing Functional Changes in Exocyst Configuration with Monoclonal Antibodies.Frontiers in cell and developmental biology · 2016Article
- The Exocyst Complex in Health and Disease.Frontiers in cell and developmental biology · 2016Review
- Distinct Roles of Cytoskeletal Components in Immunological Synapse Formation and Directed Secretion.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation.Scientific reports · 2015Article
- The Exocyst at a Glance.Journal of cell science · 2015Review
- A dynamic coordination of flagellum and cytoplasmic cytoskeleton assembly specifies cell morphogenesis in trypanosomes.Journal of cell science · 2015Article
- Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics.Scientific reports · 2015Article
- The Ca2+-activated Cl- channel ANO1/TMEM16A regulates primary ciliogenesis.Molecular biology of the cell · 2014Article
- Desmosome assembly and dynamics.Trends in cell biology · 2013Review
- Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion.The Journal of cell biology · 2013Article
- Cleavage of transmembrane junction proteins and their role in regulating epithelial homeostasis.Tissue barriers · 2013Review
- Fission yeast sec3 bridges the exocyst complex to the actin cytoskeleton.Traffic (Copenhagen, Denmark) · 2012Article
- CCAAT-enhancer-binding protein-beta expression in vivo is associated with muscle strength.Aging cell · 2012Article
- An association between type Iγ PI4P 5-kinase and Exo70 directs E-cadherin clustering and epithelial polarization.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Exocyst is a conserved multisubunit complex involved in the docking of post-Golgi transport vesicles to sites of membrane remodeling during cellular processes such as polarization, migration, and division. In mammalian epithelial cells, Exocyst complexes are recruited to nascent sites of cell-cell contact in response to E-cadherin-mediated adhesive interactions, and this event is an important early step in the assembly of intercellular junctions. Sec3 has been hypothesized to function as a spatial landmark for the development of polarity in budding yeast, but its role in epithelial cells has not been investigated. Here, we provide evidence in support of a function for a Sec3-containing Exocyst complex in the assembly or maintenance of desmosomes, adhesive junctions that link intermediate filament networks to sites of strong intercellular adhesion. We show that Sec3 associates with a subset of Exocyst complexes that are enriched at desmosomes. Moreover, we found that membrane recruitment of Sec3 is dependent on cadherin-mediated adhesion but occurs later than that of the known Exocyst components Sec6 and Sec8 that are recruited to adherens junctions. RNA interference-mediated suppression of Sec3 expression led to specific impairment of both the morphology and function of desmosomes, without noticeable effect on adherens junctions. These results suggest that two different exocyst complexes may function in basal-lateral membrane trafficking and will enable us to better understand how exocytosis is spatially organized during development of epithelial plasma membrane domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.