ArticleMolecular biology of the cell2010
Growth-limiting intracellular metabolites in yeast growing under diverse nutrient limitations.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers.
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Who cites it
131 citing papers in PubMed, 269 citations in OpenAlex.
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- Uncoupling protein production from growth: different strategies for intracellular and secreted proteins in yeast.Microbial cell factories · 2025Article
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- Coarse-Grained Modeling Elucidates Differential Metabolism ofACS synthetic biology · 2025Article
- Increased Mevalonate Production Using Engineered Citrate Synthase and Phosphofructokinase Variants of Escherichia coli.Biotechnology and bioengineering · 2025Article
- Mitochondrial ATP generation is more proteome efficient than glycolysis.Nature chemical biology · 2024Article
- An economic demand-based framework for prioritization strategies in response to transient amino acid limitations.Nature communications · 2024Article
- Phenotypic heterogeneity follows a growth-viability tradeoff in response to amino acid identity.Nature communications · 2024Article
- Emergence of Orchestrated and Dynamic Metabolism ofACS synthetic biology · 2024Article
- Understanding stoichiometric constraints on growth using resource use efficiency imbalances.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The characteristics of differentiated yeast subpopulations depend on their lifestyle and available nutrients.Scientific reports · 2024Article
- Article
- Shaping bacterial gene expression by physiological and proteome allocation constraints.Nature reviews. Microbiology · 2023Review
- The Histone Deacetylases Hst1 and Rpd3 Integrate De Novo NADInternational journal of molecular sciences · 2023Article
71 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Microbes tailor their growth rate to nutrient availability. Here, we measured, using liquid chromatography-mass spectrometry, >100 intracellular metabolites in steady-state cultures of Saccharomyces cerevisiae growing at five different rates and in each of five different limiting nutrients. In contrast to gene transcripts, where approximately 25% correlated with growth rate irrespective of the nature of the limiting nutrient, metabolite concentrations were highly sensitive to the limiting nutrient's identity. Nitrogen (ammonium) and carbon (glucose) limitation were characterized by low intracellular amino acid and high nucleotide levels, whereas phosphorus (phosphate) limitation resulted in the converse. Low adenylate energy charge was found selectively in phosphorus limitation, suggesting the energy charge may actually measure phosphorus availability. Particularly strong concentration responses occurred in metabolites closely linked to the limiting nutrient, e.g., glutamine in nitrogen limitation, ATP in phosphorus limitation, and pyruvate in carbon limitation. A simple but physically realistic model involving the availability of these metabolites was adequate to account for cellular growth rate. The complete data can be accessed at the interactive website http://growthrate.princeton.edu/metabolome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.