ArticleMolecular biology of the cell2010
Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- A suite of fluorescent timers for visualizing protein spatiotemporal dynamics in plants.Science advances · 2026Article
- Development and characterization of experimental β-cell senescence models revealing autophagy defects and altered stimulus-secretion coupling.GeroScience · 2026Article
- Molecular requirements for mammalian crinophagy highlight a key role for CaResearch square · 2025Article
- A fluorescence lifetime-based FLIM-timer for measuring the protein turnover of transcription factor Nrf2 in live cells.Scientific reports · 2025Article
- Intracellular dynamics of ubiquitin-like 3 visualized using an inducible fluorescent timer expression system.Biology open · 2024Article
- Identifying adeno-associated virus (AAV) vectors that efficiently target high grade glioma cells, for in vitro monitoring of temporal cell responses.FEBS open bio · 2024Article
- Identifying of immune-associated genes for assessing the obesity-associated risk to the offspring in maternal obesity: A bioinformatics and machine learning.CNS neuroscience & therapeutics · 2024Article
- Blue-to-Red TagFT, mTagFT, mTsFT, and Green-to-FarRed mNeptusFT2 Proteins, Genetically Encoded True and Tandem Fluorescent Timers.International journal of molecular sciences · 2023Article
- The mRubyFT Protein, Genetically Encoded Blue-to-Red Fluorescent Timer.International journal of molecular sciences · 2022Article
- Isolation and Proteomics of the Insulin Secretory Granule.Metabolites · 2021Review
- Genetically Encoded Biosensors Based on Fluorescent Proteins.Sensors (Basel, Switzerland) · 2021Review
- How old are dense-core vesicles residing inProceedings. Mathematical, physical, and engineering sciences · 2020Article
- A fluorescent timer reporter enables sorting of insulin secretory granules by age.The Journal of biological chemistry · 2020Article
- Myopic (HD-PTP, PTPN23) selectively regulates synaptic neuropeptide release.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Limited distal organelles and synaptic function in extensive monoaminergic innervation.Journal of cell science · 2017Article
- The rise of photoresponsive protein technologies applicationsF1000Research · 2017Review
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- ATP: The crucial component of secretory vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Aged insulin granules display reduced microtubule-dependent mobility and are disposed within actin-positive multigranular bodies.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Vesicle capture, not delivery, scales up neuropeptide storage in neuroendocrine terminals.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although it is evident that only a few secretory vesicles accumulating in neuroendocrine cells are qualified to fuse with the plasma membrane and release their contents to the extracellular space, the molecular mechanisms that regulate their exocytosis are poorly understood. For example, it has been controversial whether secretory vesicles are exocytosed randomly or preferentially according to their age. Using a newly developed protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO), which changes color with a predictable time course, here we show that small GTPase Rab27A effectors regulate age-dependent exocytosis of secretory vesicles in PC12 cells. When the vesicles were labeled with mK-GO-tagged neuropeptide Y or tissue-type plasminogen activator, punctate structures with green or red fluorescence were observed. Application of high [K(+)] stimulation induced exocytosis of new (green) fluorescent secretory vesicles but not of old (red) vesicles. Overexpression or depletion of rabphilin and synaptotagmin-like protein4-a (Slp4-a), which regulate exocytosis positively and negatively, respectively, disturbed the age-dependent exocytosis of the secretory vesicles in different manners. Our results suggest that coordinate functions of the two effectors of Rab27A, rabphilin and Slp4-a, are required for regulated secretory pathway.
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