ArticleJournal of virology2010
Secondary structure of the mature ex virio Moloney murine leukemia virus genomic RNA dimerization domain.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- New windows into retroviral RNA structures.Retrovirology · 2018Review
- Retroviral RNA Dimerization: From Structure to Functions.Frontiers in microbiology · 2018Review
- TMV mutants with poly(A) tracts of different lengths demonstrate structural variations in 3'UTR affecting viral RNAs accumulation and symptom expression.Scientific reports · 2015Article
- An immature retroviral RNA genome resembles a kinetically trapped intermediate state.Journal of virology · 2014Article
- Deciphering the role of the Gag-Pol ribosomal frameshift signal in HIV-1 RNA genome packaging.Journal of virology · 2014Article
- Global RNA structure analysis of poliovirus identifies a conserved RNA structure involved in viral replication and infectivity.Journal of virology · 2013Article
- Long-range architecture in a viral RNA genome.Biochemistry · 2013Article
- A guanosine-centric mechanism for RNA chaperone function.Science (New York, N.Y.) · 2013Article
- Comparison of SIV and HIV-1 genomic RNA structures reveals impact of sequence evolution on conserved and non-conserved structural motifs.PLoS pathogens · 2013Article
- Femtomole SHAPE reveals regulatory structures in the authentic XMRV RNA genome.Journal of the American Chemical Society · 2011Article
- Exploring RNA structural codes with SHAPE chemistry.Accounts of chemical research · 2011Article
- Determining the frequency and mechanisms of HIV-1 and HIV-2 RNA copackaging by single-virion analysis.Journal of virology · 2011Article
- The in vitro loose dimer structure and rearrangements of the HIV-2 leader RNA.Nucleic acids research · 2011Article
- Complex recombination patterns arising during geminivirus coinfections preserve and demarcate biologically important intra-genome interaction networks.PLoS pathogens · 2011Article
- Ensemble of secondary structures for encapsidated satellite tobacco mosaic virus RNA consistent with chemical probing and crystallography constraints.Biophysical journal · 2011Article
- Diverse interactions of retroviral Gag proteins with RNAs.Trends in biochemical sciences · 2011Review
- Structural dynamics of retroviral genome and the packaging.Frontiers in microbiology · 2011Article
- Murine leukemia viruses: objects and organisms.Advances in virology · 2011Article
- Definition of a high-affinity Gag recognition structure mediating packaging of a retroviral RNA genome.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- SHAPE-directed RNA secondary structure prediction.Methods (San Diego, Calif.) · 2010Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Retroviral genomes are dimeric, comprised of two sense-strand RNAs linked at their 5' ends by noncovalent base pairing and tertiary interactions. Viral maturation involves large-scale morphological changes in viral proteins and in genomic RNA dimer structures to yield infectious virions. Structural studies have largely focused on simplified in vitro models of genomic RNA dimers even though the relationship between these models and authentic viral RNA is unknown. We evaluate the secondary structure of the minimal dimerization domain in genomes isolated from Moloney murine leukemia virions using a quantitative and single nucleotide resolution RNA structure analysis technology (selective 2'-hydroxyl acylation analyzed by primer extension, or SHAPE). Results are consistent with an architecture in which the RNA dimer is stabilized by four primary interactions involving two sets of intermolecular base pairs and two loop-loop interactions. The dimerization domain can independently direct its own folding since heating and refolding reproduce the same structure as visualized in genomic RNA isolated from virions. Authentic ex virio RNA has a SHAPE reactivity profile similar to that of a simplified transcript dimer generated in vitro, with the important exception of a region that appears to form a compact stem-loop only in the virion-isolated RNA. Finally, we analyze the conformational changes that accompany folding of monomers into dimers in vitro. These experiments support well-defined structural models for an authentic dimerization domain and also emphasize that many features of mature genomic RNA dimers can be reproduced in vitro using properly designed, simplified RNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.