ArticleJournal of virology2010
Localization of mammalian orthoreovirus proteins to cytoplasmic factory-like structures via nonoverlapping regions of microNS.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed.
- Spatiotemporal coordination of reovirus peripheral core replication to perinuclear whole virus assembly.PLoS pathogens · 2025Article
- Molecular sociology of virus-induced cellular condensates supporting reovirus assembly and replication.Nature communications · 2024Article
- Fluorescent protein tags affect the condensation properties of a phase-separating viral protein.Molecular biology of the cell · 2024Article
- Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication.iScience · 2024Article
- Article
- Reovirus Efficiently Reassorts Genome Segments during Coinfection and Superinfection.Journal of virology · 2022Article
- Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly.PLoS pathogens · 2022Article
- Mammalian Orthoreovirus Reassortment Proceeds with Little Constraint on Segment Mixing.Journal of virology · 2022Article
- Article
- Reovirus Low-Density Particles Package Cellular RNA.Viruses · 2021Article
- Inhibition of HIF-1α accumulation in prostate cancer cells is initiated during early stages of mammalian orthoreovirus infection.Virology · 2021Article
- The reovirus μ2 C-terminal loop inversely regulates NTPase and transcription functions versus binding to factory-forming μNS and promotes replication in tumorigenic cells.Journal of virology · 2021Article
- Review
- Mammalian orthoreovirus core protein μ2 reorganizes host microtubule-organizing center components.Virology · 2020Article
- Article
- Review
- Stability of local secondary structure determines selectivity of viral RNA chaperones.Nucleic acids research · 2018Article
- Reovirus Nonstructural Protein σNS Acts as an RNA Stability Factor Promoting Viral Genome Replication.Journal of virology · 2018Article
- Nonstructural Protein σ1s Is Required for Optimal Reovirus Protein Expression.Journal of virology · 2018Article
- Characterization of a Replicating Mammalian Orthoreovirus with Tetracysteine-Tagged μNS for Live-Cell Visualization of Viral Factories.Journal of virology · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Virally induced structures called viral factories form throughout the cytoplasm of cells infected with mammalian orthoreoviruses (MRV). When expressed alone in cells, MRV nonstructural protein microNS forms factory-like structures very similar in appearance to viral factories, suggesting that it is involved in forming the structural matrix of these structures. microNS also associates with MRV core particles; the core proteins mu2, lambda1, lambda2, lambda3, and sigma2; and the RNA-binding nonstructural protein sigmaNS. These multiple associations result in the recruitment or retention of these viral proteins or particles at factory-like structures. In this study, we identified the regions of microNS necessary and sufficient for these associations and additionally examined the localization of viral RNA synthesis in infected cells. We found that short regions within the amino-terminal 220 residues of microNS are necessary for associations with core particles and necessary and sufficient for associations with the proteins mu2, lambda1, lambda2, sigma2, and sigmaNS. We also found that only the lambda3 protein associates with the carboxyl-terminal one-third of microNS and that viral RNA is synthesized within viral factories. These results suggest that microNS may act as a cytoplasmic scaffolding protein involved in localizing and coordinating viral replication or assembly intermediates for the efficient production of progeny core particles during MRV infection.
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