ReviewCurrent opinion in neurobiology2009
High-content screening of primary neurons: ready for prime time.
Review in Current opinion in neurobiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Review
- Genetically encoded cell-death indicators (GEDI) to detect an early irreversible commitment to neurodegeneration.Nature communications · 2021Article
- FORTIS: a live-cell assay to monitor AMPA receptors using pH-sensitive fluorescence tags.Translational psychiatry · 2021Article
- High-Throughput Screening Platforms in the Discovery of Novel Drugs for Neurodegenerative Diseases.Bioengineering (Basel, Switzerland) · 2021Review
- Reactive Astrocytes Contribute to Alzheimer's Disease-Related Neurotoxicity and Synaptotoxicity in a Neuron-Astrocyte Co-culture Assay.Frontiers in cellular neuroscience · 2021Article
- Live Neuron High-Content Screening Reveals Synaptotoxic Activity in Alzheimer Mouse Model Homogenates.Scientific reports · 2020Article
- On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease.Nature protocols · 2020Article
- Scaffold Ranking and Positional Scanning Identify Novel Neurite Outgrowth Promoters with Nanomolar Potency.ACS medicinal chemistry letters · 2018Article
- High Content, Phenotypic Assays and Screens for Compounds Modulating Cellular Processes in Primary Neurons.Methods in enzymology · 2018Article
- Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway.Scientific reports · 2017Article
- Nrf2 mitigates LRRK2- and α-synuclein-induced neurodegeneration by modulating proteostasis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Rapid flow cytometric measurement of protein inclusions and nuclear trafficking.Scientific reports · 2016Article
- Clinical Trials in a Dish: The Potential of Pluripotent Stem Cells to Develop Therapies for Neurodegenerative Diseases.Annual review of pharmacology and toxicology · 2016Review
- Review
- Proceedings: consideration of genetics in the design of induced pluripotent stem cell-based models of complex disease.Stem cells translational medicine · 2014Review
- Familial Alzheimer's disease modelling using induced pluripotent stem cell technology.World journal of stem cells · 2014Review
- Mutant LRRK2 toxicity in neurons depends on LRRK2 levels and synuclein but not kinase activity or inclusion bodies.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Article
- Automatic segmentation and supervised learning-based selection of nuclei in cancer tissue images.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2012Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
High-content screening (HCS), historically limited to drug-development companies, is now a powerful and affordable technology for academic researchers. Through automated routines, this technology acquires large datasets of fluorescence images depicting the functional states of thousands to millions of cells. Information on shapes, textures, intensities, and localizations is then used to create unique representations, or 'phenotypic signatures,' of each cell. These signatures quantify physiologic or diseased states, for example, dendritic arborization, drug response, or cell coping strategies. Live-cell imaging in HCS adds the ability to correlate cellular events at different points in time, thereby allowing sensitivities and observations not possible with fixed endpoint analysis. HCS with live-cell imaging therefore provides an unprecedented capability to detect spatiotemporal changes in cells and is particularly suited for time-dependent, stochastic processes such as neurodegenerative disorders.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.