ArticlePloS one2009
In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 85 citations in OpenAlex.
- Integrated Analysis of Proteomics and Metabolomics for Heat Stress in Chinese Holstein Cows.Animals : an open access journal from MDPI · 2025Article
- Myotonic dystrophy-related CDC42-binding kinase alpha (MRCKα) mediates methionine- and leucine-stimulated β-casein synthesis in bovine mammary epithelial cells via targeting mTOR.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Cellular characteristics and milk component productivity of primary bovine mammary cells for cell-cultured milk component production.Journal of animal science and technology · 2025Article
- Innate immune response of bovine mammary epithelial cells in Mycoplasma bovis mastitis using an in vitro model of bovine mammary gland infection.The Journal of veterinary medical science · 2024Article
- Current status and challenges for cell-cultured milk technology: a systematic review.Journal of animal science and biotechnology · 2024Review
- Article
- Estradiol Regulates the Expression and Secretion of Antimicrobial Peptide S100A7 via the ERK1/2-Signaling Pathway in Goat Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2022Article
- G protein-coupled receptor 30 mediates cell proliferation of goat mammary epithelial cells via MEK/ERK&PI3K/AKT signaling pathway.Cell cycle (Georgetown, Tex.) · 2022Article
- MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Effects of Essential Amino Acid Deficiency on General Control Nonderepressible 2/Eukaryotic Initiation Factor 2 Signaling and Proteomic Changes in Primary Bovine Mammary Epithelial Cells.Current issues in molecular biology · 2022Article
- Screening and Conjoint Analysis of Key lncRNAs for Milk Fat Metabolism in Dairy Cows.Frontiers in genetics · 2022Article
- Inhibition of cell proliferation and promotion of acinus-like structure formation from goat mammary epithelial cells via Wnt/β-catenin signaling.In vitro cellular & developmental biology. Animal · 2021Article
- Amino Acid Metabolomic Profiles in Bovine Mammary Epithelial Cells under Essential Amino Acid Restriction.Animals : an open access journal from MDPI · 2021Article
- Assessing extracellular vesicles from bovine mammary gland epithelial cells cultured in FBS-free medium.Extracellular vesicles and circulating nucleic acids · 2021Article
- Article
- IFN-γ Activates the TLR4-CCL5 Signaling Through Reducing Arginine Level, Leading to Enhanced Susceptibility of Bovine Mammary Epithelial Cells to Staphylococcus aureus.Inflammation · 2020Article
- Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2020Article
- Metabolomic Profiles of Bovine Mammary Epithelial Cells Stimulated by Lipopolysaccharide.Scientific reports · 2019Article
- Development and biochemical and immunological characterization of early passage and immortalized bovine intestinal epithelial cell lines from the ileum of a young calf.Cytotechnology · 2019Article
- SESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cells.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe objective of this study was to establish a culture system and elucidate the unique characteristics of a bovine mammary epithelial cell line in vitro. METHODOLOGY: Mammary tissue from a three year old lactating dairy cow (ca. 100 d relative to parturition) was used as a source of the epithelial cell line, which was cultured in collagen-coated tissue culture dishes. Fibroblasts and epithelial cells successively grew and extended from the culturing mammary tissue at the third day. Pure epithelial cells were obtained by passages culture. PRINCIPAL
findingsThe strong positive immunostaining to cytokeratin 18 suggested that the resulting cell line exhibited the specific character of epithelial cells. Epithelial cells cultured in the presence of 10% FBS, supraphysiologic concentrations of insulin, and hydrocortisone maintained a normal diploid chromosome modal number of 2n=60. Furthermore, they were capable of synthesizing beta-casein (CSN2), acetyl-CoA carboxylase-alpha (ACACA) and butyrophilin (BTN1A1). An important finding was that frozen preservation in a mixture of 90% FBS and 10% DMSO did not influence the growth characteristics, chromosome number, or protein secretion of the isolated epithelial cell line.
conclusionsThe obtained mammary epithelial cell line had normal morphology, growth characteristics, cytogenetic and secretory characteristics, thus, it might represent an useful tool for studying the function of Chinese Holstein dairy cows mammary epithelial cell (CMECs).
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