ArticlePloS one2009
Mbd3, a component of NuRD/Mi-2 complex, helps maintain pluripotency of mouse embryonic stem cells by repressing trophectoderm differentiation.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 50 citations in OpenAlex.
- Review
- BEND3 safeguards pluripotency by repressing differentiation-associated genes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Chromatin regulation and dynamics in stem cells.Current topics in developmental biology · 2020Review
- Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation.Nucleic acids research · 2018Article
- The chromatin remodeler Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes.The Journal of biological chemistry · 2017Article
- Master regulators in development: Views from the Drosophila retinal determination and mammalian pluripotency gene networks.Developmental biology · 2017Review
- MiR-134-Mbd3 axis regulates the induction of pluripotency.Journal of cellular and molecular medicine · 2016Article
- Pluripotency Factors on Their Lineage Move.Stem cells international · 2016Review
- Cdx2 efficiently induces trophoblast stem-like cells in naïve, but not primed, pluripotent stem cells.Stem cells and development · 2015Article
- The function of chromatin modifiers in lineage commitment and cell fate specification.The FEBS journal · 2015Review
- Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy.Nucleic acids research · 2015Article
- BEND3 mediates transcriptional repression and heterochromatin organization.Transcription · 2015Article
- Chromatin modifiers and remodellers: regulators of cellular differentiation.Nature reviews. Genetics · 2014Review
- Embedding the Future of Regenerative Medicine into the Open Epigenomic Landscape of Pluripotent Human Embryonic Stem Cells.Annual research & review in biology · 2013Article
- Origins and implications of pluripotent stem cell variability and heterogeneity.Nature reviews. Molecular cell biology · 2013Review
- MBD6 is a direct target of Oct4 and controls the stemness and differentiation of adipose tissue-derived stem cells.Cellular and molecular life sciences : CMLS · 2013Article
- Epigenetic regulation in pluripotent stem cells: a key to breaking the epigenetic barrier.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Nestin expression identifies ependymoma patients with poor outcome.Brain pathology (Zurich, Switzerland) · 2012Article
- Epigenetics of pluripotent cells.Acta naturae · 2012Article
- NURDs are required for diversity.The EMBO journal · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells (ES cells) can differentiate into cells derived from all three germ layers and extraembryonic tissues. While transcription factors such as, Oct4 and Nanog are well known for their requirements for undifferentiated ES cell growth, mechanisms of epigenetic repression of germ layer specific differentiation in ES cells are not well understood. Here, we investigate functions of Mbd3, a component of nucleosome remodeling and histone deacetylation complex (NuRD/Mi-2) in mouse ES cells. We find that compared to wild type ES cells, Mbd3 knockdown cells show elevated RNA expression of trophectoderm markers, including Cdx2, Eomesodermin, and Hand1. In parallel, these cells show an increased acetylation level of histone 3 in promoters of the respective genes, suggesting Mbd3 plays a role in repression of these genes in undifferentiated ES cells. However, these changes are not sufficient for definitive differentiation to trophectoderm (TE) in chimeric embryos. When further cultured in ES medium without LIF or in trophoblast stem (TS) cell medium, Mbd3 knockdown cells differentiate into TE cells, which express Cdx2 and, at later stages, trophoblast lineage specific marker Cadherin 3. These results suggest that Mbd3 helps restrict ES cells from differentiating towards the trophectoderm lineage and is an important epigenetic player in maintaining full pluripotency of mouse ES cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.