ArticleJournal of biomedicine & biotechnology2009
The roles of platelet GPIIb/IIIa and alphavbeta3 integrins during HeLa cells adhesion, migration, and invasion to monolayer endothelium under static and dynamic shear flow.
Article in Journal of biomedicine & biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Platelet‑circulating tumor cell crosstalk: A pivotal target in cancer diagnosis and therapy (Review).Oncology reports · 2026Review
- Radiotherapeutic efficacy of gold nanoparticles for high dose-rate brachytherapy compared to conventional radiotherapy: An in vitro study.Medical physics · 2025Article
- Metastasis and angiogenesis in cervical cancer: key aspects of purinergic signaling in platelets and possible therapeutic targets.Purinergic signalling · 2024Review
- Article
- Association of the human platelet antigens polymorphisms with platelet count in patients with COVID-19.Frontiers in medicine · 2023Article
- Platelets, immune cells and the coagulation cascade; friend or foe of the circulating tumour cell?Molecular cancer · 2021Review
- Identification of Novel FNIN2 and FNIN3 Fibronectin-Derived Peptides That Promote Cell Adhesion, Proliferation and Differentiation in Primary Cells and Stem Cells.International journal of molecular sciences · 2021Article
- Article
- Structure-guided design of pure orthosteric inhibitors of αIIbβ3 that prevent thrombosis but preserve hemostasis.Nature communications · 2020Article
- GRASP depletion-mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin.Molecular biology of the cell · 2019Article
- Fluid shear stress and tumor metastasis.American journal of cancer research · 2018Review
- Platelet derived TGF-β promotes cervical carcinoma cell growth by suppressing KLF6 expression.Oncotarget · 2017Article
- Article
- Investigation of the essential role of platelet-tumor cell interactions in metastasis progression using an agent-based model.Theoretical biology & medical modelling · 2014Article
- Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor.Scientific reports · 2014Article
- Notch-1 signaling promotes the malignant features of human breast cancer through NF-κB activation.PloS one · 2014Article
- VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro.International journal of nanomedicine · 2013Article
- Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?Biomicrofluidics · 2013Article
- siRNA-mediated silencing of integrin β3 expression inhibits the metastatic potential of B16 melanoma cells.Oncology reports · 2012Article
- Eptifibatide induced profound thrombocytopenia in a patient with pelvic malignancy: A case report.Gynecologic oncology case reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During their passage through the circulatory system, tumor cells undergo extensive interactions with various host cells including endothelial cells and platelets. Mechanisms mediating tumor cell adhesion, migration, and metastasis to vessel wall under flow condition are largely unknown. The aim of this study was to investigate the potential roles of GPIIb/IIIa and alphavbeta3 integrins underlying the HeLa-endothelium interaction in static and dynamic flow conditions. HeLa cell migration and invasion were studied by using Millicell cell culture insert system. The numbers of transmigrated or invaded HeLa cells significantly increased by thrombin-activated platelets and reduced by eptifibatide, a platelet inhibitor. Meanwhile, RGDWE peptides, a specific inhibitor of alphavbeta3 integrin, also inhibited HeLa cell transmigration. Interestingly, the presence of endothelial cells had significant effect on HeLa cell migration regardless of static or cocultured flow condition. The adhesion capability of HeLa cells to endothelial monolayer was also significantly affected by GPIIb/IIIa and alphavbeta3 integrins. The arrested HeLa cells increased nearly 5-fold in the presence of thrombin-activated platelets at shear stress condition (1.84 dyn/cm(2) exposure for 1 hour) than the control (static). Our findings showed that GPIIb/IIIa and alphavbeta3 integrins are important mediators in the pathology of cervical cancer and provide a molecular basis for the future therapy, and the efficient antitumor benefit should target multiple receptors on tumor cells and platelets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.