ArticlePloS one2009
AAV recombineering with single strand oligonucleotides.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- AAV vector development, back to the future.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Review
- Recombinase-Aided Amplification Combined with Lateral Flow (LF-RAA) Assay for Rapid AAV Genome Detection.ACS omega · 2022Article
- DualHuman gene therapy · 2019Article
- Article
- Adeno-associated Virus (AAV) Dual Vector Strategies for Gene Therapy Encoding Large Transgenes.The Yale journal of biology and medicine · 2017Review
- Structure-Based Designed Nano-Dysferlin Significantly Improves Dysferlinopathy in BLA/J Mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting.Frontiers in neuroscience · 2017Article
- Delivering Transgenic DNA Exceeding the Carrying Capacity of AAV Vectors.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Adeno-associated virus at 50: a golden anniversary of discovery, research, and gene therapy success--a personal perspective.Human gene therapy · 2015Review
- Adeno-associated virus inverted terminal repeats stimulate gene editing.Gene therapy · 2015Article
- Novel adeno-associated viruses derived from pig tissues transduce most major organs in mice.Scientific reports · 2014Article
- Aptamer-guided gene targeting in yeast and human cells.Nucleic acids research · 2014Article
- Dual adeno-associated virus vectors result in efficient in vitro and in vivo expression of an oversized gene, MYO7A.Human gene therapy methods · 2014Article
- Effective delivery of large genes to the retina by dual AAV vectors.EMBO molecular medicine · 2014Article
- Oversized AAV transductifon is mediated via a DNA-PKcs-independent, Rad51C-dependent repair pathway.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Viral vectors for gene delivery to the central nervous system.Neurobiology of disease · 2012Review
- Viral single-strand DNA induces p53-dependent apoptosis in human embryonic stem cells.PloS one · 2011Article
- Little vector, big gene transduction: fragmented genome reassembly of adeno-associated virus.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Adeno-associated virus (AAV) transduction initiates a signaling cascade that culminates in a transient DNA damage response. During this time, host DNA repair proteins convert the linear single-strand AAV genomes to double-strand circular monomers and concatemers in processes stimulated by the AAV inverted terminal repeats (ITRs). As the orientation of AAV genome concatemerization appears unbiased, the likelihood of concatemerization in a desired orientation is low (less than 1 in 6). Using a novel recombineering method, Oligo-Assisted AAV Genome Recombination (OAGR), this work demonstrates the ability to direct concatemerization specifically to a desired orientation in human cells. This was achieved by a single-strand DNA oligonucleotide (oligo) displaying homology to distinct AAV genomes capable of forming an intermolecular bridge for recombination. This DNA repair process results in concatemers with genomic junctions corresponding to the sequence of oligo homology. Furthermore, OAGR was restricted to single-strand, not duplexed, AAV genomes suggestive of replication-dependent recombination. Consistent with this process, OAGR demonstrated oligo polarity biases in all tested configurations except when a portion of the oligo targeted the ITR. This approach, in addition to being useful for the elucidation of intermolecular homologous recombination, may find eventual relevance for AAV mediated large gene therapy.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.