Trial reportAmerican journal of physiology. Endocrinology and metabolism2010
Skeletal muscle NAMPT is induced by exercise in humans.
Trial report in American journal of physiology. Endocrinology and metabolism, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 136 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Identification of Novel Skeletal Muscle-derived Factors That Promote Lipid Oxidation in Both Skeletal Muscle and Adipose Tissue
The Role of Visfatin Levels in Gingival Crevicular Fluid as Potential Biomarker in The Relationship Between Obesity and Periodontal Disease
Who cites it
136 citing papers in PubMed, 3 syntheses or guidelines pooled it, 295 citations in OpenAlex.
- Exercise training upregulates intracellular nicotinamide phosphoribosyltransferase expression in humans: a systematic review with meta-analysis.Frontiers in public health · 2023Pooled it
- Diagnostic value of serum cystatin C for diabetic nephropathy: a meta-analysis.BMC endocrine disorders · 2022Pooled it
- The role of cystatin C in kidney injury in children and adolescents with type 1 diabetes mellitus: a systematic review.Jornal brasileiro de nefrologiaPooled it
- Determinants of increased muscle insulin sensitivity of exercise-trained versus sedentary normal weight and overweight individuals.Science advances · 2025Trial
- Divergent serum metabolomic, skeletal muscle signaling, transcriptomic, and performance adaptations to fasted versus whey protein-fed sprint interval training.American journal of physiology. Endocrinology and metabolism · 2021Trial
- Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study.Journal of the International Society of Sports Nutrition · 2021Trial
- Molecular correlates of MRS-basedNMR in biomedicine · 2021Trial
- Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NADCell reports · 2019Trial
- Trial
- Effects of 12 Months of Caloric Restriction on Muscle Mitochondrial Function in Healthy Individuals.The Journal of clinical endocrinology and metabolism · 2017Trial
- Strengthening muscle for healthy ageing: innovative treatments for sarcopenia.Nature reviews. Drug discovery · 2026Review
- Acute exercise rewires the proteomic landscape of human immune cells.Nature communications · 2026Article
- Exercise and the hepatic sirtuin network: rethinking the research focus on SIRT1, SIRT3, and SIRT6.Frontiers in physiology · 2026Article
- NAMPT shows tissue-dependent immune-metabolic associations in osteoarthritic synovium and aging-related skeletal muscle.Frontiers in medicine · 2026Article
- NADNature metabolism · 2025Review
- The role of NADnpj metabolic health and disease · 2025Review
- Protein Acetylation and NAD+ Homeostasis in Aging Muscle.Advances in experimental medicine and biology · 2025Review
- Relationship of visfatin with obesity and osteoporosis in patients with inflammatory bowel disease: a narrative review.Frontiers in immunology · 2025Review
- Erythrocyte nicotinamide adenine dinucleotide concentration is enhanced by systematic sports participation.BMC sports science, medicine & rehabilitation · 2024Article
- Decreasing Intracellular Entropy by Increasing Mitochondrial Efficiency and Reducing ROS Formation-The Effect on the Ageing Process and Age-Related Damage.International journal of molecular sciences · 2024Review
76 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
In mammals, nicotinamide phosphoribosyltransferase (NAMPT) is responsible for the first and rate-limiting step in the conversion of nicotinamide to nicotinamide adenine dinucleotide (NAD+). NAD+ is an obligate cosubstrate for mammalian sirtuin-1 (SIRT1), a deacetylase that activates peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha), which in turn can activate mitochondrial biogenesis. Given that mitochondrial biogenesis is activated by exercise, we hypothesized that exercise would increase NAMPT expression, as a potential mechanism leading to increased mitochondrial content in muscle. A cross-sectional analysis of human subjects showed that athletes had about a twofold higher skeletal muscle NAMPT protein expression compared with sedentary obese, nonobese, and type 2 diabetic subjects (P < 0.05). NAMPT protein correlated with mitochondrial content as estimated by complex III protein content (R(2) = 0.28, P < 0.01), MRS-measured maximal ATP synthesis (R(2) = 0.37, P = 0.002), and Vo(2max) (R(2) = 0.63, P < 0.0001). In an exercise intervention study, NAMPT protein increased by 127% in sedentary nonobese subjects after 3 wk of exercise training (P < 0.01). Treatment of primary human myotubes with forskolin, a cAMP signaling pathway activator, resulted in an approximately 2.5-fold increase in NAMPT protein expression, whereas treatment with ionomycin had no effect. Activation of AMPK via AICAR resulted in an approximately 3.4-fold increase in NAMPT mRNA (P < 0.05) as well as modest increases in NAMPT protein (P < 0.05) and mitochondrial content (P < 0.05). These results demonstrate that exercise increases skeletal muscle NAMPT expression and that NAMPT correlates with mitochondrial content. Further studies are necessary to elucidate the pathways regulating NAMPT as well as its downstream effects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.