ArticleEndocrinology2010
Rerouting of a follicle-stimulating hormone analog to the regulated secretory pathway.
Article in Endocrinology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- Pituitary gonadotroph-specific patterns of gene expression and hormone secretion.Current opinion in pharmacology · 2022Review
- Mouse models for the analysis of gonadotropin secretion and action.Best practice & research. Clinical endocrinology & metabolism · 2018Review
- Fshb Knockout Mouse Model, Two Decades Later and Into the Future.Endocrinology · 2018Review
- Induction of Stress Signaling In Vitro and Suppression of Gonadotropin Secretion by Free Fatty Acids in Female Mouse Gonadotropes.Endocrinology · 2018Article
- Redirecting intracellular trafficking and the secretion pattern of FSH dramatically enhances ovarian function in mice.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A novel carboxyl-terminal heptapeptide initiates the regulated secretion of LH from unique sub-domains of the ER.PloS one · 2013Article
- Synthesis and secretion of gonadotropins including structure-function correlates.Reviews in endocrine & metabolic disorders · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
LH and FSH are produced by the same gonadotrope cells of the anterior pituitary but differ in their mode of secretion. This coordinated secretion of LH and FSH is essential for normal follicular development and ovulation in females and for spermatogenesis in males. The structural signals encoded in the LH and FSH subunits that govern the intracellular sorting of LH through the regulated secretory pathway and FSH through the constitutive pathway are largely unknown. Our laboratory recently identified the seven amino acid carboxy tail of LH beta as a sorting signal for LH in GH(3) cells. Here we compared the morphological features of GH(3) cells expressing an FSH analog containing the heptapeptide (FL7AA) with wild-type FSH using confocal microscopy. These experiments were performed to develop a rerouting model for examining structure-function links between secretion pathways of FSH/LH and their biological action. Both FSH- and LH-expressing cells exhibit a fluorescence pattern of randomly dispersed cytoplasmic puncta. FL7AA expressing cells have more intracellular accumulation compared with wild-type FSH and display a unique halo pattern of fluorescence near the plasma membrane. Such a pattern was not observed in cells expressing FSH or LH. Our results demonstrate that this FSH analog containing the carboxy heptapeptide of LH beta is rerouted to the regulated secretory pathway in GH(3) cells. This rerouted gonadotropin provides a unique model to study the trafficking, regulation, and function of LH and FSH.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.