ArticleThe Journal of biological chemistry2010
The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Article
- Electrostatic Interactions between CSTF2 and pre-mRNA Drive Cleavage and Polyadenylation.Biophysical journal · 2022Article
- Alternative polyadenylation: methods, mechanism, function, and role in cancer.Journal of experimental & clinical cancer research : CR · 2021Review
- A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans.Nucleic acids research · 2020Article
- A modified clear-native polyacrylamide gel electrophoresis technique to investigate the oligomeric state of MBP-5-HTProtein expression and purification · 2019Article
- The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity.Nucleic acids research · 2018Article
- Useful Bicistronic Reporter System for Studying Poly(A) Site-Defining cis Elements and Regulation of Alternative Polyadenylation.International journal of molecular sciences · 2018Article
- The Cstf2t Polyadenylation Gene Plays a Sex-Specific Role in Learning Behaviors in Mice.PloS one · 2016Article
- CstF-64 is necessary for endoderm differentiation resulting in cardiomyocyte defects.Stem cell research · 2014Article
- A large number of nuclear genes in the human parasite blastocystis require mRNA polyadenylation to create functional termination codons.Genome biology and evolution · 2014Article
- Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.Molecular and cellular biology · 2014Review
- Structure and function of pre-mRNA 5'-end capping quality control and 3'-end processing.Biochemistry · 2014Article
- The stem-loop luciferase assay for polyadenylation (SLAP) method for determining CstF-64-dependent polyadenylation activity.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Article
- mRNA 3' end processing factors: a phylogenetic comparison.Comparative and functional genomics · 2012Article
- Interactions of CstF-64, CstF-77, and symplekin: implications on localisation and function.Molecular biology of the cell · 2011Article
- Pre-mRNA 3'-end processing complex assembly and function.Wiley interdisciplinary reviews. RNAReview
- Structural biology of poly(A) site definition.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Because polyadenylation is essential for cell growth, in vivo examination of polyadenylation protein function has been difficult. Here we describe a new in vivo assay that allows structure-function assays on CstF-64, a protein that binds to pre-mRNAs downstream of the cleavage site for accurate and efficient polyadenylation. In this assay (the stem-loop luciferase assay for polyadenylation, SLAP), expression of a luciferase pre-mRNA with a modified downstream sequence element was made dependent upon co-expression of an MS2-CstF-64 fusion protein. We show here that SLAP accurately reflects CstF-64-dependent polyadenylation, confirming the validity of this assay. Using SLAP, we determined that CstF-64 domains involved in RNA binding, interaction with CstF-77 (the "Hinge" domain), and coupling to transcription are critical for polyadenylation. Further, we showed that the Hinge domain is necessary for CstF-64 interaction with CstF-77 and consequent nuclear localization, suggesting that nuclear import of a preformed CstF complex is an essential step in polyadenylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.