ArticleThe Journal of biological chemistry2010
Characterization of a bifunctional pyranose-furanose mutase from Campylobacter jejuni 11168.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 35 citations in OpenAlex.
- Biosynthesis of CDP-α-d-fucofuranose and CDP-β-l-6-deoxy-altrofuranose for the Capsular Polysaccharides ofBiochemistry · 2025Article
- Identification and Functional Characterization of the Polymerizing Glycosyltransferase Required for the Transfer of d-Ribose to the d-GalBiochemistry · 2025Article
- Identification of the Polymerizing Glycosyltransferase Required for the Addition of d-Glucuronic Acid to the Capsular Polysaccharide ofBiochemistry · 2025Article
- Virulence factors and antimicrobial resistance profiles ofMicrobiology spectrum · 2023Article
- Pan-Genome-Wide Association Study of Serotype 19A Pneumococci Identifies Disease-Associated Genes.Microbiology spectrum · 2023Article
- Broiler house environment and litter management practices impose selective pressures on antimicrobial resistance genes and virulence factors ofbioRxiv : the preprint server for biology · 2023Article
- Functional Characterization of a HAD Phosphatase Involved in Capsular Polysaccharide Biosynthesis inBiochemistry · 2022Article
- Efficient Synthesis of UDP-Furanoses via 4,5-Dicyanoimidazole(DCI)-Promoted Coupling of Furanosyl-1-Phosphates with Uridine Phosphoropiperidate.Molecules (Basel, Switzerland) · 2019Article
- Deciphering the sugar biosynthetic pathway and tailoring steps of nucleoside antibiotic A201A unveils a GDP-l-galactose mutase.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Complete Tetrasaccharide Repeat Unit Biosynthesis of the Immunomodulatory Bacteroides fragilis Capsular Polysaccharide A.ACS chemical biology · 2017Article
- Chalcone Isomerase from Eubacterium ramulus Catalyzes the Ring Contraction of Flavanonols.Journal of bacteriology · 2016Article
- Article
- Gene function hypotheses for the Campylobacter jejuni glycome generated by a logic-based approach.Journal of molecular biology · 2013Article
- Elucidation of substrate specificity in Aspergillus nidulans UDP-galactose-4-epimerase.PloS one · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
UDP-galactopyranose mutases (UGM) are the enzymes responsible for the synthesis of UDP-galactofuranose (UDP-Galf) from UDP-galactopyranose (UDP-Galp). The enzyme, encoded by the glf gene, is present in bacteria, parasites, and fungi that express Galf in their glycoconjugates. Recently, a UGM homologue encoded by the cj1439 gene has been identified in Campylobacter jejuni 11168, an organism possessing no Galf-containing glycoconjugates. However, the capsular polysaccharide from this strain contains a 2-acetamido-2-deoxy-d-galactofuranose (GalfNAc) moiety. Using an in vitro high performance liquid chromatography assay and complementation studies, we characterized the activity of this UGM homologue. The enzyme, which we have renamed UDP-N-acetylgalactopyranose mutase (UNGM), has relaxed specificity and can use either UDP-Gal or UDP-GalNAc as a substrate. Complementation studies of mutase knock-outs in C. jejuni 11168 and Escherichia coli W3110, the latter containing Galf residues in its lipopolysaccharide, demonstrated that the enzyme recognizes both UDP-Gal and UDP-GalNAc in vivo. A homology model of UNGM and site-directed mutagenesis led to the identification of two active site amino acid residues involved in the recognition of the UDP-GalNAc substrate. The specificity of UNGM was characterized using a two-substrate co-incubation assay, which demonstrated, surprisingly, that UDP-Gal is a better substrate than UDP-GalNAc.
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