ArticleProceedings of the National Academy of Sciences of the United States of America2009
Direct observation of twisting steps during Rad51 polymerization on DNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- SWI5-SFR1 reduces RAD51 recombinase extending units during filament assembly.Nucleic acids research · 2025Article
- Single-molecule fluorescence imaging techniques reveal molecular mechanisms underlying deoxyribonucleic acid damage repair.Frontiers in bioengineering and biotechnology · 2022Review
- Observation and Analysis of RAD51 Nucleation Dynamics at Single-Monomer Resolution.Methods in enzymology · 2018Article
- Visualization and quantification of nascent RAD51 filament formation at single-monomer resolution.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Telomeric overhang length determines structural dynamics and accessibility to telomerase and ALT-associated proteins.Structure (London, England : 1993) · 2014Article
- Gold rotor bead tracking for high-speed measurements of DNA twist, torque and extension.Nature methods · 2014Article
- Structural and torsional properties of the RAD51-dsDNA nucleoprotein filament.Nucleic acids research · 2013Article
- Probing Rad51-DNA interactions by changing DNA twist.Nucleic acids research · 2012Article
- Recent developments in single-molecule DNA mechanics.Current opinion in structural biology · 2012Review
- Single-molecule views of protein movement on single-stranded DNA.Annual review of biophysics · 2012Review
- Freely orbiting magnetic tweezers to directly monitor changes in the twist of nucleic acids.Nature communications · 2011Article
- Real-time observation of strand exchange reaction with high spatiotemporal resolution.Structure (London, England : 1993) · 2011Article
- Stepwise translocation of nucleic acid motors.Current opinion in structural biology · 2010Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human recombinase hRad51 is a key protein for the maintenance of genome integrity and for cancer development. Polymerization and depolymerization of hRad51 on duplex DNA were studied here using a new generation of magnetic tweezers, measuring DNA twist in real time with a resolution of 5 degrees . Our results combined with earlier structural information suggest that DNA is somewhat less extended by hRad51 than by RecA (4.5 vs. 5.1 A per base pair) and untwisted by 18.2 degrees per base pair. They also confirm a stoichiometry of 3-4 bp per protein in the hRad51-dsDNA nucleoprotein filament. At odds with earlier claims, we show that after initial deposition of a multimeric nucleus, nucleoprotein filament growth occurs by addition/release of single proteins, involving DNA twisting steps of 65 degrees +/- 5 degrees. Simple numeric simulations show that this mechanism is an efficient way to minimize nucleoprotein filament defects. Nucleoprotein filament growth from a preformed nucleus was observed at hRad51 concentrations down to 10 nM, whereas nucleation was never observed below 100 nM in the same buffer. This behavior can be associated with the different stoichiometries of nucleation and growth. It may be instrumental in vivo to permit efficient continuation of strand exchange by hRad51 alone while requiring additional proteins such as Rad52 for its initiation, thus keeping the latter under the strict control of regulatory pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.