ArticleAntimicrobial agents and chemotherapy2010
Screening and quantification of the expression of antibiotic resistance genes in Acinetobacter baumannii with a microarray.
Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 121 citations in OpenAlex.
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- Contribution of RND superfamily multidrug efflux pumps AdeABC, AdeFGH, and AdeIJK to antimicrobial resistance and virulence factors in multidrug-resistantAntimicrobial agents and chemotherapy · 2025Article
- C7-Substituted Quinolines as Potent Inhibitors of AdeG Efflux Pumps inACS infectious diseases · 2025Article
- Detection of AdeAB, TetA, and TetB efflux pump genes in clinical isolates of tetracycline-resistant Acinetobacter baumannii from patients of Suez Canal University Hospitals.BMC microbiology · 2025Article
- Alteration in the Morphological and Transcriptomic Profiles ofMicroorganisms · 2024Article
- First Characterization ofViruses · 2024Article
- Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria.Diagnostics (Basel, Switzerland) · 2023Review
- The Mechanism of Tigecycline Resistance inInternational journal of molecular sciences · 2023Article
- RND pumps across the genusMicrobial genomics · 2023Article
- The RND Efflux Pump Gene Expression in the Biofilm Formation ofAntibiotics (Basel, Switzerland) · 2023Article
- Resistance mechanisms of tigecycline inFrontiers in cellular and infection microbiology · 2023Review
- Integron Functionality and Genome Innovation: An Update on the Subtle and Smart Strategy of Integrase and Gene Cassette Expression Regulation.Microorganisms · 2022Review
- Identification of Promoter Region Markers Associated With Altered Expression of Resistance-Nodulation-Division Antibiotic Efflux Pumps inFrontiers in microbiology · 2022Article
- Minocycline Susceptibility andInfection and drug resistance · 2022Article
- Evaluation the effect of ZnO nanoparticle derivedJournal of environmental health science & engineering · 2021Article
- Antimicrobial Resistance Gene Detection Methods for Bacteria in Animal-Based Foods: A Brief Review of Highlights and Advantages.Microorganisms · 2021Review
- Characteristics and diversity of mutations in regulatory genes of resistance-nodulation-cell division efflux pumps in association with drug-resistant clinical isolates of Acinetobacter baumannii.Antimicrobial resistance and infection control · 2021Article
- Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by IntracellularMicroorganisms · 2021Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An oligonucleotide-based DNA microarray was developed to evaluate expression of genes for efflux pumps in Acinetobacter baumannii and to detect acquired antibiotic resistance determinants. The microarray contained probes for 205 genes, including those for 47 efflux systems, 55 resistance determinants, and 35 housekeeping genes. The microarray was validated by comparative analysis of mutants overexpressing or deficient in the pumps relative to the parental strain. The performance of the microarray was also evaluated using in vitro single-step mutants obtained on various antibiotics. Overexpression, confirmed by quantitative reverse transcriptase PCR, of RND efflux pumps AdeABC, due to a G30D substitution in AdeS in a multidrug-resistant (MDR) strain obtained on gentamicin, and AdeIJK, in two mutants obtained on cefotaxime or tetracycline, was detected. A new efflux pump, AdeFGH, was found to be overexpressed in a mutant obtained on chloramphenicol. Study of MDR clinical isolates, including the AYE strain, whose entire sequence has been determined, indicated overexpression of AdeABC and of the chromosomally encoded cephalosporinase as well as the presence of several acquired resistance genes. The overexpressed and acquired determinants detected by the microarray could account for nearly the entire MDR phenotype of the isolates. The microarray is potentially useful for detection of resistance in A. baumannii and should allow detection of new efflux systems associated with antibiotic resistance.
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