ArticleMolecular and cellular biology2010
Tagged mutagenesis by efficient Minos-based germ line transposition.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Murine allele and transgene symbols: ensuring unique, concise, and informative nomenclature.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- Inducible protein traps with dominant phenotypes for functional analysis of the Drosophila genome.Genetics · 2014Article
- Normalizing electrocardiograms of both healthy persons and cardiovascular disease patients for biometric authentication.PloS one · 2013Article
- High-throughput semiquantitative analysis of insertional mutations in heterogeneous tumors.Genome research · 2011Article
- Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells.Nature protocols · 2011Article
- The Drosophila gene disruption project: progress using transposons with distinctive site specificities.Genetics · 2011Article
- Article
- Harnessing transposons for cancer gene discovery.Nature reviews. Cancer · 2010Review
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Germ line gene transposition technology has been used to generate "libraries" of flies and worms carrying genomewide mutations. Phenotypic screening and DNA sequencing of such libraries provide functional information resulting from insertional events in target genes. There is also a great need to have a fast and efficient way to generate mouse mutants in vivo to model developmental defects and human diseases. Here we describe an optimized mammalian germ line transposition system active during early mouse spermatogenesis using the Minos transposon. Transposon-positive progeny carry on average more than 2 new transpositions, and 45 to 100% of the progeny carry an insertion in a gene. The optimized Minos-based system was tested in a small rapid dominant functional screen to identify mutated genes likely to cause measurable cardiovascular "disease" phenotypes in progeny/embryos. Importantly this system allows rapid screening for modifier genes.
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Registered trials
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