ArticleGenetics2010
Sen1p performs two genetically separable functions in transcription and processing of U5 small nuclear RNA in Saccharomyces cerevisiae.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Sen1 architecture: RNA-DNA hybrid resolution, autoregulation, and insights into SETX inactivation in AOA2.Molecular cell · 2023Article
- Modulated termination of non-coding transcription partakes in the regulation of gene expression.Nucleic acids research · 2022Article
- Article
- Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage.Nature communications · 2018Article
- Senataxin Mutation Reveals How R-Loops Promote Transcription by Blocking DNA Methylation at Gene Promoters.Molecular cell · 2018Article
- Biochemical characterization of the helicase Sen1 provides new insights into the mechanisms of non-coding transcription termination.Nucleic acids research · 2017Article
- Nucleoside Triphosphate Phosphohydrolase I (NPH I) Functions as a 5' to 3' Translocase in Transcription Termination of Vaccinia Early Genes.The Journal of biological chemistry · 2016Article
- Pcf11 orchestrates transcription termination pathways in yeast.Genes & development · 2015Article
- Saccharomyces cerevisiae Sen1 as a model for the study of mutations in human Senataxin that elicit cerebellar ataxia.Genetics · 2014Article
- Mutation of senataxin alters disease-specific transcriptional networks in patients with ataxia with oculomotor apraxia type 2.Human molecular genetics · 2014Article
- Human snRNA genes use polyadenylation factors to promote efficient transcription termination.Nucleic acids research · 2014Article
- Exome sequencing of a patient with suspected mitochondrial disease reveals a likely multigenic etiology.BMC medical genetics · 2013Article
- A bacterial-like mechanism for transcription termination by the Sen1p helicase in budding yeast.Nature structural & molecular biology · 2013Article
- The conserved C-terminus of the PcrA/UvrD helicase interacts directly with RNA polymerase.PloS one · 2013Article
- Sen1p contributes to genomic integrity by regulating expression of ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae.PloS one · 2013Article
- Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response.Molecular and cellular biology · 2013Article
- Disengaging polymerase: terminating RNA polymerase II transcription in budding yeast.Biochimica et biophysica acta · 2013Review
- Interactions of Sen1, Nrd1, and Nab3 with multiple phosphorylated forms of the Rpb1 C-terminal domain in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- The PolyA tail length of yeast histone mRNAs varies during the cell cycle and is influenced by Sen1p and Rrp6p.Nucleic acids research · 2012Article
- Emerging Views on the CTD Code.Genetics research international · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae SEN1 gene codes for a nuclear-localized superfamily I helicase. SEN1 is an ortholog of human SETX (senataxin), which has been implicated in the neurological disorders ataxia-ocular apraxia type 2 and juvenile amyotrophic lateral sclerosis. Pleiotropic phenotypes conferred by sen1 mutations suggest that Sen1p affects multiple steps in gene expression. Sen1p is embedded in a protein-protein interaction network involving direct binding to multiple partners. To test whether the interactions occur independently or in a dependent sequence, we examined interactions with the RNA polymerase II subunit Rpb1p, which is required for transcription, and Rnt1p, which is required for 3'-end maturation of many noncoding RNAs. Mutations were identified that impair one of the two interactions without impairing the other interaction. The effects of the mutants on the synthesis of U5 small nuclear RNA were analyzed. Two defects were observed, one in transcription termination and one in 3'-end maturation. Impairment of the Sen1p-Rpb1p interaction resulted in a termination defect. Impairment of the Sen1p-Rnt1p interaction resulted in a processing defect. The results suggest that the Sen1p-Rpb1p and Sen1p-Rnt1p interactions occur independently of each other and serve genetically separable purposes in targeting Sen1p to function in two temporally overlapping steps in gene expression.
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