ArticleBiophysical journal2009
Application of fluorescence spectroscopy to quantify shear-induced protein conformation change.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Loss of pulsatility with continuous-flow left ventricular assist devices and the significance of the arterial endothelium in von-Willebrand factor production and degradation.Artificial organs · 2023Review
- Turbulent Flow Promotes Cleavage of VWF (von Willebrand Factor) by ADAMTS13 (A Disintegrin and Metalloproteinase With a Thrombospondin Type-1 Motif, Member 13).Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain.Blood advances · 2019Article
- Total chemical synthesis and biophysical properties of a designed soluble 24 kDa amyloid analogue.Chemical science · 2018Article
- Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants.Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2018Article
- Platelet activation risk index as a prognostic thrombosis indicator.Scientific reports · 2016Article
- Review
- Role of fluid shear stress in regulating VWF structure, function and related blood disorders.Biorheology · 2015Review
- Biophysical Studies on BEX3, the p75NTR-Associated Cell Death Executor, Reveal a High-Order Oligomer with Partially Folded Regions.PloS one · 2015Article
- The cytotoxicity of BAMLET complexes is due to oleic acid and independent of the α-lactalbumin component.FEBS open bio · 2015Article
- Platelet GpIba binding to von Willebrand Factor under fluid shear:contributions of the D′D3-domain, A1-domain flanking peptide and O-linked glycans.Journal of the American Heart Association · 2014Article
- Escherichia coli-derived von Willebrand factor-A2 domain fluorescence/Förster resonance energy transfer proteins that quantify ADAMTS13 activity.Analytical biochemistry · 2011Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Rapid and robust methods are required to quantify the effect of hydrodynamic shear on protein conformation change. We evaluated such strategies in this work and found that the binding of the fluorescent probe 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid (bis-ANS) to hydrophobic pockets in the blood protein von Willebrand factor (VWF) is enhanced upon the application of fluid shear to the isolated protein. Significant structural changes were observed when the protein was sheared at shear rates >or= 6000/s for approximately 3.5 min. The binding of bis-ANS to multimeric VWF, but not dimeric VWF or control protein bovine serum albumin, was enhanced upon fluid shear application. Thus, high-molecular-weight VWF is more susceptible to conformation change upon tensile loading. Although bis-ANS itself did not alter the conformation of VWF, it stabilized protein conformation once it bound the sheared molecule. Bis-ANS binding to VWF was reduced when the sheared protein was allowed to relax before dye addition. Taken together with functional data in the literature, our results suggest that shear-induced conformation changes in VWF reported by bis-ANS correlate well with the normal function of the protein under physiological/pathological fluid flow conditions. Further, this study introduces the fluorescent dye bis-ANS as a tool that may be useful in studies of shear-induced protein conformation change.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.