ArticleNature structural & molecular biology2009
Single-molecule analysis of protein-free U2-U6 snRNAs.
Article in Nature structural & molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 65 citations in OpenAlex.
- Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP.Genome biology · 2025Article
- Mapping snoRNA-target RNA interactions in an RNA binding protein-dependent manner with chimeric eCLIP.bioRxiv : the preprint server for biology · 2024Article
- Impact of bisphenol-A on the spliceosome and meiosis of sperm in the testis of adolescent mice.BMC veterinary research · 2022Article
- Role of the central junction in folding topology of the protein-free human U2-U6 snRNA complex.RNA (New York, N.Y.) · 2020Article
- DSP1 and DSP4 Act Synergistically in Small Nuclear RNA 3' End Maturation and Pollen Growth.Plant physiology · 2019Article
- TDP-43 regulates site-specific 2'-O-methylation of U1 and U2 snRNAs via controlling the Cajal body localization of a subset of C/D scaRNAs.Nucleic acids research · 2019Article
- Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.Chemical reviews · 2018Review
- Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells.Nature communications · 2018Article
- Methodologies for studying the spliceosome's RNA dynamics with single-molecule FRET.Methods (San Diego, Calif.) · 2017Review
- Article
- Biological Activities of Extracellular Vesicles and Their Cargos from Bovine and Human Milk in Humans and Implications for Infants.The Journal of nutrition · 2017Review
- Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.Wiley interdisciplinary reviews. RNA · 2016Review
- What have single-molecule studies taught us about gene expression?Genes & development · 2016Review
- Single molecule analysis reveals reversible and irreversible steps during spliceosome activation.eLife · 2016Article
- Global genome splicing analysis reveals an increased number of alternatively spliced genes with aging.Aging cell · 2016Article
- Genome-wide identification and functional prediction of novel and fungi-responsive lincRNAs in Triticum aestivum.BMC genomics · 2016Article
- Conformational dynamics of stem II of the U2 snRNA.RNA (New York, N.Y.) · 2016Article
- An active site rearrangement within the Tetrahymena group I ribozyme releases nonproductive interactions and allows formation of catalytic interactions.RNA (New York, N.Y.) · 2016Article
- Differential Assembly of Catalytic Interactions within the Conserved Active Sites of Two Ribozymes.PloS one · 2016Article
- Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Spliceosomes catalyze the maturation of precursor mRNAs in organisms ranging from yeast to humans. Their catalytic core comprises three small nuclear RNAs (U2, U5 and U6) involved in substrate positioning and catalysis. It has been postulated, but never shown experimentally, that the U2-U6 complex adopts at least two conformations that reflect different activation states. We have used single-molecule fluorescence to probe the structural dynamics of a protein-free RNA complex modeling U2-U6 from yeast and mutants of highly conserved regions of U2-U6. Our data show the presence of at least three distinct conformations in equilibrium. The minimal folding pathway consists of a two-step process with an obligatory intermediate. The first step is strongly magnesium dependent, and we provide evidence suggesting that the second step corresponds to the formation of the genetically conserved helix IB. Site-specific mutations in the highly conserved AGC triad and the U80 base in U6 suggest that the observed conformational dynamics correlate with residues that have an important role in splicing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.