ArticleEukaryotic cell2009
Algal lipid bodies: stress induction, purification, and biochemical characterization in wild-type and starchless Chlamydomonas reinhardtii.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers.
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Who cites it
166 citing papers in PubMed.
- Identification and functional analysis of class 3 lipases associated with triacylglycerol metabolism in Nannochloropsis oceanica (Eustigmatophyceae).Journal of phycology · 2026Article
- DpAGPs overexpression enhanced application value of Dunaliella Parva for bioethanol production.World journal of microbiology & biotechnology · 2026Article
- Genome-Wide Characterization and Expression Analysis ofBiology · 2025Article
- Remodeling of the terpenoid metabolism during prolonged phosphate depletion in the marine diatom Phaeodactylum tricornutum.Journal of phycology · 2025Article
- Chlamydomonas reinhardtii, Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores.The Plant journal : for cell and molecular biology · 2025Article
- The proteome is a terminal electron acceptor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Engineered Chlorella vulgaris improves bioethanol production and promises prebiotic application.World journal of microbiology & biotechnology · 2024Article
- Increasing lipid production inFrontiers in bioengineering and biotechnology · 2024Article
- Comparison of the effects of nitrogen-, sulfur- and combined nitrogen- and sulfur-deprivations on cell growth, lipid bodies and gene expressions in Chlamydomonas reinhardtii cc5373-sta6.BMC biotechnology · 2023Article
- Exploiting AGPase genes and encoded proteins to prioritize development of optimum engineered strains in microalgae towards sustainable biofuel production.World journal of microbiology & biotechnology · 2023Article
- COPlants (Basel, Switzerland) · 2022Article
- Physiological and Genetic Regulation for High Lipid Accumulation byMicrobiology spectrum · 2022Article
- Article
- PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis.Plant physiology · 2022Article
- Macular pigment-enriched oil production from genome-edited microalgae.Microbial cell factories · 2022Article
- Article
- Recycling of the major thylakoid lipid MGDG and its role in lipid homeostasis in Chlamydomonas reinhardtii.Plant physiology · 2021Article
- Underwater Light Manipulation by the Benthic DiatomNanomaterials (Basel, Switzerland) · 2021Article
- Assessment on the oil accumulation by knockdown of triacylglycerol lipase in the oleaginous diatom Fistulifera solaris.Scientific reports · 2021Article
- Lipid droplet evolution gives insight into polyaneuploid cancer cell lipid droplet functions.Medical oncology (Northwood, London, England) · 2021Review
106 more citing papers are in PubMed but not listed here.
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
When the unicellular green soil alga Chlamydomonas reinhardtii is deprived of nitrogen after entering stationary phase in liquid culture, the cells produce abundant cytoplasmic lipid bodies (LBs), as well as abundant starch, via a pathway that accompanies a regulated autophagy program. After 48 h of N starvation in the presence of acetate, the wild-type LB content has increased 15-fold. When starch biosynthesis is blocked in the sta6 mutant, the LB content increases 30-fold, demonstrating that genetic manipulation can enhance LB production. The use of cell wall-less strains permitted development of a rapid "popped-cell" microscopic assay to quantitate the LB content per cell and permitted gentle cell breakage and LB isolation. The highly purified LBs contain 90% triacylglycerol (TAG) and 10% free fatty acids (FFA). The fatty acids associated with the TAGs are approximately 50% saturated (C(16) and C(18)) fatty acids and approximately 50% unsaturated fatty acids, half of which are in the form of oleic acid (C(18:1)). The FFA are approximately 50% C(16) and approximately 50% C(18). The LB-derived TAG yield from a liter of sta6 cells at 10(7) cells/ml after starvation for 48 h is calculated to approach 400 mg. The LB fraction also contains low levels of charged glycerolipids, with the same profile as whole-cell charged glycerolipids, that presumably form LB membranes; chloroplast-specific neutral glycerolipids (galactolipids) are absent. Very low levels of protein are also present, but all matrix-assisted laser desorption ionization-identified species are apparent contaminants. Nitrogen stress-induced LB production in C. reinhardtii has the hallmarks of a discrete pathway that should be amenable to additional genetic and culture condition manipulation.
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