ArticleApplied and environmental microbiology2010
Differential expression of virulence and stress fitness genes between Escherichia coli O157:H7 strains with clinical or bovine-biased genotypes.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Factors Governing the Cross-Species Virulence of Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2026Article
- Clinical Outcomes and Virulence Factors of Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2024Article
- Pathogenomes and variations in Shiga toxin production among geographically distinct clones ofMicrobial genomics · 2022Article
- Longitudinal Study of Shiga Toxin-ProducingApplied and environmental microbiology · 2019Article
- Factors Involved in the Persistence of a Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification.Electronic physician · 2016Article
- Diversity of Survival Patterns among Escherichia coli O157:H7 Genotypes Subjected to Food-Related Stress Conditions.Frontiers in microbiology · 2016Article
- Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC).PloS one · 2015Article
- Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers.Open forum infectious diseases · 2014Article
- Microarray analysis of the Ler regulon in enteropathogenic and enterohaemorrhagic Escherichia coli strains.PloS one · 2014Article
- Lineage and genogroup-defining single nucleotide polymorphisms of Escherichia coli O157:H7.Applied and environmental microbiology · 2013Article
- Multilocus genotype analysis of Escherichia coli O157 isolates from Australia and the United States provides evidence of geographic divergence.Applied and environmental microbiology · 2013Article
- Characterization of Escherichia coli O157:H7 strains isolated from supershedding cattle.Applied and environmental microbiology · 2013Article
- Differences in virulence gene expression between atypical enteropathogenic Escherichia coli strains isolated from diarrheic and healthy ruminants.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2013Article
- Evolution of the Stx2-encoding prophage in persistent bovine Escherichia coli O157:H7 strains.Applied and environmental microbiology · 2013Article
- Variation in stress resistance patterns among stx genotypes and genetic lineages of shiga toxin-producing Escherichia coli O157.Applied and environmental microbiology · 2012Article
- Genetic features differentiating bovine, food, and human isolates of shiga toxin-producing Escherichia coli O157 in The Netherlands.Journal of clinical microbiology · 2012Article
- Distinct transcriptional profiles and phenotypes exhibited by Escherichia coli O157:H7 isolates related to the 2006 spinach-associated outbreak.Applied and environmental microbiology · 2012Article
- Carriage of stx2a differentiates clinical and bovine-biased strains of Escherichia coli O157.PloS one · 2012Article
- Differential virulence of clinical and bovine-biased enterohemorrhagic Escherichia coli O157:H7 genotypes in piglet and Dutch belted rabbit models.Infection and immunity · 2012Article
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Authors and funding
5 authors.
Funding
Abstract
Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genotypes). To determine whether inherent differences in gene expression explain the variation in infectivity of these genotypes, we compared the expression patterns of clinical genotype 1 strains with those of bovine-biased genotype 5 strains using microarrays. Important O157:H7 virulence factors, including locus of enterocyte effacement genes, the enterohemolysin, and several pO157 genes, showed increased expression in the clinical versus bovine-biased genotypes. In contrast, genes essential for acid resistance (e.g., gadA, gadB, and gadC) and stress fitness were upregulated in bovine-biased genotype 5 strains. Increased expression of acid resistance genes was confirmed functionally using a model stomach assay, in which strains of bovine-biased genotype 5 had a 2-fold-higher survival rate than strains of clinical genotype 1. Overall, these results suggest that the increased prevalence of O157:H7 illness caused by clinical genotype 1 strains is due in part to the overexpression of key virulence genes. The bovine-biased genotype 5 strains, however, are more resistant to adverse environmental conditions, a characteristic that likely facilitates O157:H7 colonization of bovines.
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