ArticleJournal of bacteriology2010
HspR mutations are naturally selected in Bifidobacterium longum when successive heat shock treatments are applied.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Review
- Stress Response in Bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2022Review
- Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria.Applied and environmental microbiology · 2022Article
- Transcriptomic analysis of Bifidobacterium longum subsp. longum BBMN68 in response to oxidative shock.Scientific reports · 2018Article
- A Commensal Bifidobacterium longum Strain Prevents Gluten-Related Immunopathology in Mice through Expression of a Serine Protease Inhibitor.Applied and environmental microbiology · 2017Article
- Overexpression of Small Heat Shock Protein Enhances Heat- and Salt-Stress Tolerance of Bifidobacterium longum NCC2705.Current microbiology · 2015Article
- Expression of fluorescent proteins in bifidobacteria for analysis of host-microbe interactions.Applied and environmental microbiology · 2014Article
- Enhancing probiotic stability in industrial processes.Microbial ecology in health and disease · 2012Article
- Enhancing the stress responses of probiotics for a lifestyle from gut to product and back again.Microbial cell factories · 2011Review
- How do bifidobacteria counteract environmental challenges? Mechanisms involved and physiological consequences.Genes & nutrition · 2011Article
- Genomic insights into bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2010Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of molecular tools allowed light to be shed on several widespread genetic mechanisms aiming at limiting the effect of molecular damage on bacterial survival. For some bacterial taxa, there are limited tools in the genetic toolbox, which restricts the possibilities to investigate the molecular basis of their stress response. In that case, an alternative strategy is to study genetic variants of a strain under stress conditions. The comparative study of the genetic determinants responsible for their phenotypes, e.g., an improved tolerance to stress, offers precious clues on the molecular mechanisms effective in this bacterial taxon. We applied this approach and isolated two heat shock-tolerant strains derived from Bifidobacterium longum NCC2705. A global analysis of their transcriptomes revealed that the dnaK operon and the clpB gene were overexpressed in both heat shock-tolerant strains. We sequenced the hspR gene coding for the negative regulator of dnaK and clpB and found point mutations affecting protein domains likely responsible for the binding of the regulators to the promoter DNA. Complementation of the mutant strains by the wild-type regulator hspR restored its heat sensitivity and thus demonstrated that these mutations were responsible for the observed heat tolerance phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.