ArticleJournal of bacteriology2010
Thermococcus kodakarensis mutants deficient in di-myo-inositol phosphate use aspartate to cope with heat stress.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Resource partitioning and amino acid assimilation in a terrestrial geothermal spring.The ISME journal · 2023Article
- Archaea as a Model System for Molecular Biology and Biotechnology.Biomolecules · 2023Review
- Solutions: how adaptive changes in cellular fluids enable marine life to cope with abiotic stressors.Marine life science & technology · 2022Review
- An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.Folia microbiologica · 2020Review
- Cross-Stress Adaptation in a Piezophilic and Hyperthermophilic Archaeon From Deep Sea Hydrothermal Vent.Frontiers in microbiology · 2020Article
- Random mutagenesis of a hyperthermophilic archaeon identified tRNA modifications associated with cellular hyperthermotolerance.Nucleic acids research · 2019Article
- Molecular chaperone accumulation as a function of stress evidences adaptation to high hydrostatic pressure in the piezophilic archaeon Thermococcus barophilus.Scientific reports · 2016Article
- Identification of N(α)-acetyl-α-lysine as a probable thermolyte and its accumulation mechanism in Salinicoccus halodurans H3B36.Scientific reports · 2015Article
- Proteome profiling of heat, oxidative, and salt stress responses in Thermococcus kodakarensis KOD1.Frontiers in microbiology · 2015Article
- Mannosylglycerate and di-myo-inositol phosphate have interchangeable roles during adaptation of Pyrococcus furiosus to heat stress.Applied and environmental microbiology · 2014Article
- An archaeal glutamate decarboxylase homolog functions as an aspartate decarboxylase and is involved in β-alanine and coenzyme A biosynthesis.Journal of bacteriology · 2014Article
- DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction.Frontiers in microbiology · 2014Article
- An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity.The Journal of biological chemistry · 2013Article
- Genetics Techniques for Thermococcus kodakarensis.Frontiers in microbiology · 2012Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
- Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts.Microbial biotechnology · 2011Review
- Molecular bases of thermophily in hyperthermophiles.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2011Review
- Production, crystallization and preliminary X-ray analysis of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many of the marine microorganisms which are adapted to grow at temperatures above 80 degrees C accumulate di-myo-inositol phosphate (DIP) in response to heat stress. This led to the hypothesis that the solute plays a role in thermoprotection, but there is a lack of definitive experimental evidence. Mutant strains of Thermococcus kodakarensis (formerly Thermococcus kodakaraensis), manipulated in their ability to synthesize DIP, were constructed and used to investigate the involvement of DIP in thermoadaptation of this archaeon. The solute pool of the parental strain comprised DIP, aspartate, and alpha-glutamate. Under heat stress the level of DIP increased 20-fold compared to optimal conditions, whereas the pool of aspartate increased 4.3-fold in response to osmotic stress. Deleting the gene encoding the key enzyme in DIP synthesis, CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase, abolished DIP synthesis. Conversely, overexpression of the same gene resulted in a mutant with restored ability to synthesize DIP. Despite the absence of DIP in the deletion mutant, this strain exhibited growth parameters similar to those of the parental strain, both at optimal (85 degrees C) and supraoptimal (93.7 degrees C) temperatures for growth. Analysis of the respective solute pools showed that DIP was replaced by aspartate. We conclude that DIP is part of the strategy used by T. kodakarensis to cope with heat stress, and aspartate can be used as an alternative solute of similar efficacy. This is the first study using mutants to demonstrate the involvement of compatible solutes in the thermoadaptation of (hyper)thermophilic organisms.
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