ArticleExperimental physiology2010
Vascular endothelial growth factor enhances cancer cell adhesion to microvascular endothelium in vivo.
Article in Experimental physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
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- Differential effects of vascular endothelial growth factor on glycocalyx of endothelial and tumor cells and potential targets for tumor metastasis.APL bioengineering · 2022Article
- The Glycocalyx and Its Role in Vascular Physiology and Vascular Related Diseases.Cardiovascular engineering and technology · 2021Review
- Tuning Cancer Fate: Tumor Microenvironment's Role in Cancer Stem Cell Quiescence and Reawakening.Frontiers in immunology · 2020Review
- Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis.Journal of extracellular vesicles · 2019Article
- Metastatic niche functions and therapeutic opportunities.Nature cell biology · 2018Review
- Quantification of Malignant Breast Cancer Cell MDA-MB-231 Transmigration Across Brain and Lung Microvascular Endothelium.Annals of biomedical engineering · 2016Article
- Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows.American journal of physiology. Heart and circulatory physiology · 2016Article
- Sphingosine-1-phosphate Maintains Normal Vascular Permeability by Preserving Endothelial Surface Glycocalyx in Intact Microvessels.Microcirculation (New York, N.Y. : 1994) · 2016Article
- Reinforcing endothelial junctions prevents microvessel permeability increase and tumor cell adhesion in microvessels in vivo.Scientific reports · 2015Article
- Endothelial surface glycocalyx can regulate flow-induced nitric oxide production in microvessels in vivo.PloS one · 2015Article
- Secreted meningeal chemokines, but not VEGFA, modulate the migratory properties of medulloblastoma cells.Biochemical and biophysical research communications · 2014Article
- Differential arrest and adhesion of tumor cells and microbeads in the microvasculature.Biomechanics and modeling in mechanobiology · 2014Article
- Microvascular transport and tumor cell adhesion in the microcirculation.Annals of biomedical engineering · 2012Review
- Adhesion of malignant mammary tumor cells MDA-MB-231 to microvessel wall increases microvascular permeability via degradation of endothelial surface glycocalyx.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
- Quantification of the endothelial surface glycocalyx on rat and mouse blood vessels.Microvascular research · 2012Article
- Effects of wall shear stress and its gradient on tumor cell adhesion in curved microvessels.Biomechanics and modeling in mechanobiology · 2012Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
To investigate whether vascular endothelial growth factor (VEGF) enhances cancer cell adhesion to normal microvessels, we used in vivo video microscopy to measure adhesion rates of MDA-MB-435s human breast cancer cells and ErbB2-transformed mouse mammary carcinomas in the postcapillary venules of rat mesentery. An individual postcapillary venule in the mesentery was injected via a glass micropipette with cancer cells either in a perfusate of mammalian Ringer solution containing 1% bovine serum albumin as a control, or with the addition of 1 nm VEGF for test measurements. Cell adhesion was measured as either the number of adherent cells or the fluorescence intensity of adherent cells in a vessel segment for 60 min. Our results showed that during both control and VEGF treatments, the number of adherent cells increased almost linearly with time over 60 min. The VEGF treatment increased the adhesion rates of human tumour cells and mouse carcinomas 1.9-fold and 1.8-fold, respectively, over those in control conditions. We also measured cancer cell adhesion after pretreatment of cells with an antibody blocking VEGF or an antibody blocking alpha 6 integrin, and pretreatment of the microvessel with VEGF receptor (KDR/Flk-1) inhibitor, SU1498, or anti-integrin extracellular matrix ligand antibody, anti-laminin-5. All antibodies and inhibitor significantly reduced adhesion, with anti-VEGF and SU1498 reducing it the most. Our results indicate that VEGF enhances cancer cell adhesion to the normal microvessel wall, and further suggest that VEGF and its receptor, KDR/Flk-1, as well as integrins of tumour cells and their ligands at the endothelium, contribute to mammary cancer cell adhesion to vascular endothelium in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.