ArticleBiomaterials2010
Structure-activity relationships of cationic shell-crosslinked knedel-like nanoparticles: shell composition and transfection efficiency/cytotoxicity.
Article in Biomaterials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
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- Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment.Beilstein journal of nanotechnology · 2025Review
- Urchin-like ceria nanoparticles for enhanced gene therapy of osteoarthritis.Science advances · 2023Article
- Development of Fully Degradable Phosphonium-Functionalized Amphiphilic Diblock Copolymers for Nucleic Acids Delivery.Biomacromolecules · 2018Article
- In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging.Biomaterials · 2016Article
- Ultra-pH-sensitive nanoprobe library with broad pH tunability and fluorescence emissions.Journal of the American Chemical Society · 2014Article
- Liposomal spherical nucleic acids.Journal of the American Chemical Society · 2014Article
- PEGylation of cationic, shell-crosslinked-knedel-like nanoparticles modulates inflammation and enhances cellular uptake in the lung.Nanomedicine : nanotechnology, biology, and medicine · 2013Article
- Cytokines as biomarkers of nanoparticle immunotoxicity.Chemical Society reviews · 2013Review
- Antisense peptide nucleic acid-functionalized cationic nanocomplex for in vivo mRNA detection.Interface focus · 2013Article
- Degradable cationic shell cross-linked knedel-like nanoparticles: synthesis, degradation, nucleic acid binding, and in vitro evaluation.Biomacromolecules · 2013Article
- Efficient protection and transfection of small interfering RNA by cationic shell-crosslinked knedel-like nanoparticles.Nucleic acid therapeutics · 2013Article
- Endosomal escape and siRNA delivery with cationic shell crosslinked knedel-like nanoparticles with tunable buffering capacities.Biomaterials · 2012Article
- Antibody-linked spherical nucleic acids for cellular targeting.Journal of the American Chemical Society · 2012Article
- 64Cu Core-labeled nanoparticles with high specific activity via metal-free click chemistry.ACS nano · 2012Article
- Strategies Toward Well-Defined Polymer Nanoparticles Inspired by Nature: Chemistry versus Versatility.Journal of polymer science. Part A, Polymer chemistry · 2012Article
- Design of polymeric nanoparticles for biomedical delivery applications.Chemical Society reviews · 2012Review
- Dual peptide nucleic acid- and peptide-functionalized shell cross-linked nanoparticles designed to target mRNA toward the diagnosis and treatment of acute lung injury.Bioconjugate chemistry · 2012Article
- The importance of chemistry in creating well-defined nanoscopic embedded therapeutics: devices capable of the dual functions of imaging and therapy.Accounts of chemical research · 2011Review
- Polyvalent nucleic acid nanostructures.Journal of the American Chemical Society · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Cationic nanoparticles are a promising class of transfection agents for oligonucleotide and gene delivery, but vary greatly in their effectiveness and cytotoxicity. Recently, we developed a new class of cationic transfection agents based on cationic shell-crosslinked knedel-like nanoparticles (cSCKs) that efficiently transfect mammalian cells with both oligonucleotides and plasmid DNA. In an effort to further improve transfection efficiency without increasing cytotoxicity, we examined the effects of the composition of primary amine (pa), tertiary amine (ta) and carboxylic acid (ca) groups in the shell of these nanoparticles. A series of discrete complexes of the cSCKs with plasmid DNA (pDNA) or phosphorothioate 2'-OMe oliogonucleotides (ps-MeON) were prepared over a broad range of amine to phosphate ratios (N/P ratio) of 4:1-40:1. The sizes of the complexes and the ability of the nanoparticles to completely bind ODNs were found to depend on the cSCK amine to DNA phosphate (N/P) ratio and the cSCK buffering capacity. The cSCKs were then evaluated for their ability to transfect cells with plasmid DNA by monitoring fluorescence from an encoded EGFP, and for delivery of ps-MeON by monitoring luminescence from luciferase resulting from ps-MeON-mediated splicing correction. Whereas the cationic cSCK-pa(25)-ta(75) was found to be best for transfecting plasmid DNA into HeLa cells at an N/P ratio of 20:1, cSCK-pa(50)-ta(50), at an N/P ratio 10:1 was best for ps-MeON delivery. We also found that increasing the proportion of tertiary relative to primary amine reduced the cytotoxicity. These results demonstrate that a dramatic improvement in gene and oligonucleotide delivery efficiency with decreased cytotoxicity in HeLa cells can be achieved by incorporation of tertiary amines into the shells of cSCKs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.