ArticleJournal of molecular biology2010
Mechanistic role of structurally dynamic regions in Dicistroviridae IGR IRESs.
Article in Journal of molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- RNAViruses · 2026Review
- Genetic mechanisms underlying the structural elaboration and dissemination of viral internal ribosomal entry sites.Nucleic acids research · 2026Article
- Article
- Comprehensive mutational analysis of the sequence-function relationship within a viral internal ribosome entry site.Nucleic acids research · 2025Article
- Initiation of translation on nedicistrovirus and related intergenic region IRESs by their factor-independent binding to the P site of 80S ribosomes.RNA (New York, N.Y.) · 2023Article
- The Halastavi árva Virus Intergenic Region IRES Promotes Translation by the Simplest Possible Initiation Mechanism.Cell reports · 2020Article
- UGA stop codon readthrough to translate intergenic region of Plautia stali intestine virus does not require RNA structures forming internal ribosomal entry site.RNA (New York, N.Y.) · 2019Article
- Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.eLife · 2016Article
- A dynamic RNA loop in an IRES affects multiple steps of elongation factor-mediated translation initiation.eLife · 2015Article
- Tricks an IRES uses to enslave ribosomes.Trends in microbiology · 2012Review
- Host translation at the nexus of infection and immunity.Cell host & microbe · 2012Review
- Insights into factorless translational initiation by the tRNA-like pseudoknot domain of a viral IRES.PloS one · 2012Article
- In vivo functional analysis of the Dicistroviridae intergenic region internal ribosome entry sites.Nucleic acids research · 2011Article
- Activity of the human immunodeficiency virus type 1 cell cycle-dependent internal ribosomal entry site is modulated by IRES trans-acting factors.Nucleic acids research · 2011Article
- HCV IRES domain IIb affects the configuration of coding RNA in the 40S subunit's decoding groove.RNA (New York, N.Y.) · 2011Article
- SHAPE-directed RNA secondary structure prediction.Methods (San Diego, Calif.) · 2010Review
- Modular domains of the Dicistroviridae intergenic internal ribosome entry site.RNA (New York, N.Y.) · 2010Article
- The structures of nonprotein-coding RNAs that drive internal ribosome entry site function.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Dicistroviridae intergenic region (IGR) internal ribosome entry site(s) (IRES) RNAs drive a cap-independent pathway of translation initiation, recruiting both small and large ribosomal subunits to viral RNA without the use of any canonical translation initiation factors. This ability is conferred by the folded three-dimensional structure of the IRES RNA, which has been solved by X-ray crystallography. Here, we report the chemical probing of Plautia stali intestine virus IGR IRES in the unbound form, in the 40S-subunit-bound form, and in the 80S-ribosome-bound form. The results, when combined with an analysis of crystal structures, suggest that parts of the IRES RNA change structure as the preinitiation complex forms. Using mutagenesis coupled with native gel electrophoresis, preinitiation complex assembly assays, and translation initiation assays, we show that these potentially structurally dynamic elements of the IRES are involved in different steps in the pathway of ribosome recruitment and translation initiation. Like tRNAs, it appears that the IGR IRES undergoes local structural changes that are coordinated with structural changes in the ribosome, and these are critical for the IRES mechanism of action.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.