ArticleBMC molecular biology2009
Evaluation of real-time PCR endogenous control genes for analysis of gene expression in bovine endometrium.
Article in BMC molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 90 citations in OpenAlex.
- Enrichment ofInternational journal of molecular sciences · 2024Article
- Generation of a novel three-dimensional scaffold-based model of the bovine endometrium.Veterinary research communications · 2023Article
- Successful editing and maintenance of lactogenic gene expression in primary bovine mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2023Article
- Identification of stable reference genes for quantitative gene expression analysis in the duodenum of meat-type ducks.Frontiers in veterinary science · 2023Article
- Reference gene selection in bovine caruncular epithelial cells under pregnancy-associated hormones exposure.Scientific reports · 2022Article
- Identification of stably expressed reference genes for expression studies inFrontiers in plant science · 2022Article
- RANBP10 promotes glioblastoma progression by regulating the FBXW7/c-Myc pathway.Cell death & disease · 2021Article
- Prediction of Calving to Conception Interval Length Using Algorithmic Analysis of Endometrial mRNA Expression in Bovine.Animals : an open access journal from MDPI · 2021Article
- Comparative Transcriptome Analysis of Milk Somatic Cells During Lactation Between Two Intensively Reared Dairy Sheep Breeds.Frontiers in genetics · 2021Article
- Article
- Supplementing Ca salts of soybean oil after artificial insemination increases pregnancy success in Bos taurus beef cows.Journal of animal science · 2018Article
- Deep sequencing of the transcriptome in the anterior pituitary of heifers before and after ovulation.The Journal of veterinary medical science · 2017Article
- Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy.International journal of molecular sciences · 2017Article
- Differential gene expression profiling of endometrium during the mid-luteal phase of the estrous cycle between a repeat breeder (RB) and non-RB cows.Reproductive biology and endocrinology : RB&E · 2017Article
- Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro.Reproductive biology and endocrinology : RB&E · 2016Article
- mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages.Reproductive biology and endocrinology : RB&E · 2016Article
- URI1 amplification in uterine carcinosarcoma associates with chemo-resistance and poor prognosis.American journal of cancer research · 2015Article
- Discovery of a bovine enterovirus in alpaca.PloS one · 2013Article
- Reference gene selection for real-time quantitative PCR analysis of the mouse uterus in the peri-implantation period.PloS one · 2013Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitative real-time PCR gene expression results are generally normalised using endogenous control genes. These reference genes should be expressed at a constant level across all sample groups in a study, and should not be influenced by study treatments or conditions. There has been no systematic investigation of endogenous control genes for bovine endometrium to date. The suitability of both commonly used and novel endogenous control genes was evaluated in this study, with the latter being selected from stably expressed transcripts identified through microarray analysis of bovine endometrium. Fifteen candidate endogenous control genes were assessed across different tissue subtypes in pregnant and cycling Holstein-Friesian dairy cows from two divergent genetic backgrounds.
resultsThe expression profiles of five commonly used endogenous control genes (GAPDH, PPIA, RPS9, RPS15A, and UXT) and 10 experimentally derived candidate endogenous control genes (SUZ12, C2ORF29, ZNF131, ACTR1A, HDAC1, SLC30A6, CNOT7, DNAJC17, BBS2, and RANBP10) were analysed across 44 samples to determine the most stably expressed gene. Gene stability was assessed using the statistical algorithms GeNorm and Normfinder. All genes presented with low overall variability (0.87 to 1.48% CV of Cq). However, when used to normalise a differentially expressed gene (oxytocin receptor - OXTR) in the samples, the reported relative gene expression levels were significantly affected by the control gene chosen. Based on the results of this analysis, SUZ12 is proposed as the most appropriate control gene for use in bovine endometrium during early pregnancy or the oestrus cycle.
conclusionThis study establishes the suitability of novel endogenous control genes for comparing expression levels in endometrial tissues of pregnant and cycling bovines, and demonstrates the utility of microarray analysis as a method for identifying endogenous control gene candidates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.