ArticleMicrobial cell factories2009
High yield recombinant thermostable alpha-amylase production using an improved Bacillus licheniformis system.
Article in Microbial cell factories, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Overcoming cellular secretion bottlenecks: advanced secretion engineering and molecular tailoring for next-generation microbial α-amylases with enhanced industrial performance.World journal of microbiology & biotechnology · 2026Review
- Bacillaceae in Probiotics and Postbiotics: Applications, Functional Traits, Mechanisms and Outlook.Probiotics and antimicrobial proteins · 2026Review
- EndophyticMicrobiology spectrum · 2026Article
- Recombinant Forms of α-Amylase AmyBL159 from a Thermophilic BacteriumMicroorganisms · 2025Article
- Engineered β-galactosidase catalyzes lactose to prebiotics in situ in raw milk.Biotechnology letters · 2025Article
- Synergistic Engineering of the Twin-Arginine Translocation (Tat) Pathway and Membrane Capacity Enhances Extracellular Production of Amylosucrase inMicroorganisms · 2025Article
- Enhancing β-Galactosidase Performance for Galactooligosaccharides Preparation via Strategic Glucose Re-Tunneling.International journal of molecular sciences · 2024Article
- Secretory expression of amylosucrase in Bacillus licheniformis through twin-arginine translocation pathway.Journal of industrial microbiology & biotechnology · 2024Article
- Pullulanase with high temperature and low pH optima improved starch saccharification efficiency.Scientific reports · 2022Article
- High-efficiency chromosomal integrative amplification strategy for overexpressing α-amylase in Bacillus licheniformis.Journal of industrial microbiology & biotechnology · 2022Article
- Microbial production of small peptide: pathway engineering and synthetic biology.Microbial biotechnology · 2021Review
- Exploitation of ammonia-inducible promoters for enzyme overexpression in Bacillus licheniformis.Journal of industrial microbiology & biotechnology · 2021Article
- Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus.Microbial cell factories · 2020Article
- Development of Bacillus amyloliquefaciens as a high-level recombinant protein expression system.Journal of industrial microbiology & biotechnology · 2019Article
- A new strategy to express the extracellular α-amylase from Pyrococcus furiosus in Bacillus amyloliquefaciens.Scientific reports · 2016Article
- Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression.Microbial cell factories · 2015Article
- Microbial interactions for enhancement of α-amylase production byBiotechnology reports (Amsterdam, Netherlands) · 2014Article
- Amylolytic microorganism from são paulo zoo composting: isolation, identification, and amylase production.Enzyme research · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSome strains of Bacillus licheniformis have been improved by target-directed screening as well as by classical genetic manipulation and used in commercial thermostable alpha-amylase and alkaline protease production for over 40 years. Further improvements in production of these enzymes are desirable.
resultsA new strain of B. licheniformis CBBD302 carrying a recombinant plasmid pHY-amyL for Bacillus licheniformis alpha-amylase (BLA) production was constructed. The combination of target-directed screening and genetic recombination led to an approximately 26-fold improvement of BLA production and export in B. licheniformis. Furthermore, a low-cost fermentation medium containing soybean meal and cottonseed meal for BLA production in shake-flasks and in a 15 liter bioreactor was developed and a BLA concentration of up to 17.6 mg per ml growth medium was attained.
conclusionThis production level of BLA by B. licheniformis CBBD302(pHY-amyL) is amongst the highest levels in Gram-positive bacteria reported so far.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.