ArticleArchives of microbiology2009
Identification, cloning, and functional characterization of EmrD-3, a putative multidrug efflux pump of the major facilitator superfamily from Vibrio cholerae O395.
Article in Archives of microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- New Cross-Linked Polymeric Materials Modified with Antimicrobial Compounds in Relation to Their Biological Activities and Biodegradation by the Laccase-Producing FungusBiomolecules · 2026Article
- Antibiotic Resistance in Acetic Acid Bacteria Originating from Vinegar.Antibiotics (Basel, Switzerland) · 2024Article
- Emerging resistome diversity in clinical Vibrio cholerae strains revealing their role as potential reservoirs of antimicrobial resistance.Molecular biology reports · 2024Article
- Major facilitator superfamily efflux pumps in human pathogens: Role in multidrug resistance and beyond.Current research in microbial sciences · 2024Article
- Efflux pumps as potential targets for biofilm inhibition.Frontiers in microbiology · 2024Review
- Perturbation of Quorum Sensing after the Acquisition of Bacteriophage Resistance Could Contribute to Novel Traits inMicroorganisms · 2023Article
- Inhibition of Multidrug Efflux Pumps Belonging to the Major Facilitator Superfamily in Bacterial Pathogens.Biomedicines · 2023Review
- Article
- Membrane Efflux Pumps of PathogenicMicroorganisms · 2022Review
- Antibiotic Potentiators Against Multidrug-Resistant Bacteria: Discovery, Development, and Clinical Relevance.Frontiers in microbiology · 2022Review
- Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.Chemical reviews · 2021Review
- Novel Soil-Derived Beta-Lactam, Chloramphenicol, Fosfomycin and Trimethoprim Resistance Genes Revealed by Functional Metagenomics.Antibiotics (Basel, Switzerland) · 2021Article
- Shedding light on the bacterial resistance to toxic UV filters: a comparative genomic study.PeerJ · 2021Article
- Role of McbR in the regulation of antibiotic susceptibility in avian pathogenic Escherichia coli.Poultry science · 2020Article
- Synergistic Activity of Colistin-Containing Combinations against Colistin-Resistant Enterobacteriaceae.Antimicrobial agents and chemotherapy · 2018Article
- Modulation of antimicrobial efflux pumps of the major facilitator superfamily inAIMS microbiology · 2018Review
- The Food Production Environment and the Development of Antimicrobial Resistance in Human Pathogens of Animal Origin.Microorganisms · 2017Review
- RNA-seq-based analysis of drug-resistant Salmonella enterica serovar Typhimurium selected in vivo and in vitro.PloS one · 2017Article
- Mycobacterium smegmatis MSMEG_3705 encodes a selective major facilitator superfamily efflux pump with multiple roles.Current microbiology · 2015Article
- The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.Clinical microbiology reviews · 2015Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A putative multidrug efflux pump, EmrD-3, belonging to the major facilitator superfamily (MFS) of transporters and sharing homology with the Bcr/CflA subfamily, was identified in Vibrio cholerae O395. We cloned the emrD-3 gene and evaluated its role in antimicrobial efflux in a hypersensitive Escherichia coli strain. The efflux activity of this membrane protein resulted in lowering the intracellular concentration of ethidium. The recombinant plasmid carrying emrD-3 conferred enhanced resistance to several antimicrobials. Among the antimicrobials tested, the highest relative increase in minimum inhibitory concentration (MIC) of 102-fold was observed for linezolid (MIC = 256 microg/ml), followed by an 80.1-fold increase for tetraphenylphosphonium chloride (TPCL) (156.2 microg/ml), 62.5-fold for rifampin (MIC = 50 microg/ml), >30-fold for erythromycin (MIC = 50 microg/ml) and minocycline (MIC = 2 microg/ml), 20-fold for trimethoprim (MIC = 0.12 microg/ml), and 18.7-fold for chloramphenicol (MIC = 18.7 microg/ml). Among the fluorescent DNA-binding dyes, the highest relative increase in MIC of 41.7-fold was observed for ethidium bromide (125 microg/ml) followed by a 17.2-fold increase for rhodamine 6G (100 microg/ml). Thus, we demonstrate that EmrD-3 is a multidrug efflux pump of V. cholerae, the homologues of which are present in several Vibrio spp., some members of Enterobacteriaceae family, and Gram-positive Bacillus spp.
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