ArticleNature protocols2009
Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- A Method for Isolation and Transfection of Arabidopsis Protoplast for Sucrose Feeding Assay.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Establishing Reagent Testing Platforms for Functional Analyses in Sunflower.Plants (Basel, Switzerland) · 2025Article
- Agrobacterium-mediated transient transformation of Flaveria bidentis leaves: a novel method to examine the evolution of CPlant methods · 2024Article
- A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves.Plant methods · 2024Article
- Genetic engineering of novel yellow color african violet (Saintpaulia ionantha) produced by accumulation of Aureusidin 6-O-glucoside.Biological procedures online · 2022Article
- Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress.Scientific reports · 2021Article
- An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.BMC plant biology · 2021Article
- Screening of a multi-virus resistant RNAi construct in cowpea through transient vacuum infiltration method.Virusdisease · 2019Article
- Resequencing of cv CRI-12 family reveals haplotype block inheritance and recombination of agronomically important genes in artificial selection.Plant biotechnology journal · 2019Article
- Article
- Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants.Journal of visualized experiments : JoVE · 2018Article
- Article
- Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells.PloS one · 2017Article
- A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding inPlant methods · 2017Article
- Once for All: A Novel Robust System for Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in Plants.Frontiers in plant science · 2017Article
- A sunflower WRKY transcription factor stimulates the mobilization of seed-stored reserves during germination and post-germination growth.Plant cell reports · 2016Article
- Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis.BMC biotechnology · 2016Article
- RXLR and CRN Effectors from the Sunflower Downy Mildew PathogenFrontiers in plant science · 2016Article
- Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana.Plant cell reports · 2015Article
- Development and optimization of agroinfiltration for soybean.Plant cell reports · 2015Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries and efficient and rapid transformation protocols. In a wide variety of species, Agrobacterium-mediated transient transformation is a useful tool that can provide valuable insight into many biological processes. However, this technology has not been routinely applied to the sunflower because of difficulties with infiltration. Here, we present an optimized protocol for Agrobacterium-mediated transient transformation of leaf discs. Using this procedure, we were able to quickly overexpress or silence a given gene, enabling us to study several biochemical processes and characterize sunflower regulatory sequences. One of the major advantages of this approach is that in only 1 work-week it is possible to acquire considerable molecular information while avoiding the use of controversial heterologous systems. Transforming heterologous species is frequently unacceptable, as the conservation of molecular events in many cases is not well documented.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.