ArticleThe Journal of biological chemistry2009
Thiolated tRNAs of Trypanosoma brucei are imported into mitochondria and dethiolated after import.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Remodeling of tRNA modification in Trypanosoma cruzi life forms.PLoS pathogens · 2026Article
- Article
- tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei.Nucleic acids research · 2021Article
- How the intracellular partitioning of tRNA and tRNA modification enzymes affects mitochondrial function.IUBMB life · 2018Review
- Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- Divergence of Erv1-associated mitochondrial import and export pathways in trypanosomes and anaerobic protists.Eukaryotic cell · 2013Article
- The absence of A-to-I editing in the anticodon of plant cytoplasmic tRNA (Arg) ACG demands a relaxation of the wobble decoding rules.RNA biology · 2012Article
- Determinants of tRNA editing and modification: avoiding conundrums, affecting function.Seminars in cell & developmental biology · 2012Review
- In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.EMBO reports · 2011Article
- Assembling Fe/S-clusters and modifying tRNAs: ancient co-factors meet ancient adaptors.Trends in parasitology · 2011Article
- Futile import of tRNAs and proteins into the mitochondrion of Trypanosoma brucei evansi.Molecular and biochemical parasitology · 2011Article
- The Fe/S cluster assembly protein Isd11 is essential for tRNA thiolation in Trypanosoma brucei.The Journal of biological chemistry · 2010Article
- Transfer RNA modifications: nature's combinatorial chemistry playground.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All mitochondrial tRNAs in Trypanosoma brucei derive from cytosolic tRNAs that are in part imported into mitochondria. Some trypanosomal tRNAs are thiolated in a compartment-specific manner. We have identified three proteins required for the thio modification of cytosolic tRNA(Gln), tRNA(Glu), and tRNA(Lys). RNA interference-mediated ablation of these proteins results in the cytosolic accumulation non-thio-modified tRNAs but does not increase their import. Moreover, in vitro import experiments showed that both thio-modified and non-thio-modified tRNA(Glu) can efficiently be imported into mitochondria. These results indicate that unlike previously suggested the cytosol-specific thio modifications do not function as antideterminants for mitochondrial tRNA import. Consistent with these results we showed by using inducible expression of a tagged tRNA(Glu) that it is mainly the thiolated form that is imported in vivo. Unexpectedly, the imported tRNA becomes dethiolated after import, which explains why the non-thiolated form is enriched in mitochondria. Finally, we have identified two genes required for thiolation of imported tRNA(Trp) whose wobble nucleotide is subject to mitochondrial C to U editing. Interestingly, down-regulation of thiolation resulted in an increase of edited tRNA(Trp) but did not affect growth.
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