ArticleNucleic acids research2010
Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Detecting gene expression in Caenorhabditis elegans.Genetics · 2025Review
- Behavioral adjustment of C. elegans to mechanosensory loss requires intact mechanosensory neurons.PLoS biology · 2024Article
- Molecular encoding and synaptic decoding of context during salt chemotaxis in C. elegans.Nature communications · 2022Article
- G protein-coupled receptors as candidates for modulation and activation of the chemical senses in decapod crustaceans.PloS one · 2021Article
- Article
- Single cell transcriptomes reveal expression patterns of chemoreceptor genes in olfactory sensory neurons of the Caribbean spiny lobster, Panulirus argus.BMC genomics · 2020Article
- Cell Type-specific mRNA Purification inBio-protocol · 2019Article
- Comprehensive single-cell transcriptional profiling of a multicellular organism.Science (New York, N.Y.) · 2017Article
- Article
- Analysis of C. elegans muscle transcriptome using trans-splicing-based RNA tagging (SRT).Nucleic acids research · 2016Article
- Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor.Nature communications · 2016Article
- Feeding state-dependent regulation of developmental plasticity via CaMKI and neuroendocrine signaling.eLife · 2015Article
- Identification of Wnt Pathway Target Genes Regulating the Division and Differentiation of Larval Seam Cells and Vulval Precursor Cells in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2015Article
- Comparative RNA-Seq analysis reveals pervasive tissue-specific alternative polyadenylation in Caenorhabditis elegans intestine and muscles.BMC biology · 2015Article
- Getting Down to Specifics: Profiling Gene Expression and Protein-DNA Interactions in a Cell Type-Specific Manner.Advances in genetics · 2015Review
- Genomics and proteomics in solving brain complexity.Molecular bioSystems · 2013Review
- Insights into post-transcriptional regulation during legume-rhizobia symbiosis.Plant signaling & behavior · 2013Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The nervous system is composed of a wide variety of neurons. A description of the transcriptional profiles of each neuron would yield enormous information about the molecular mechanisms that define morphological or functional characteristics. Here we show that RNA isolation from single neurons is feasible by using an optimized mRNA tagging method. This method extracts transcripts in the target cells by co-immunoprecipitation of the complexes of RNA and epitope-tagged poly(A) binding protein expressed specifically in the cells. With this method and genome-wide microarray, we compared the transcriptional profiles of two functionally different neurons in the main C. elegans gustatory neuron class ASE. Eight of the 13 known subtype-specific genes were successfully detected. Additionally, we identified nine novel genes including a receptor guanylyl cyclase, secreted proteins, a TRPC channel and uncharacterized genes conserved among nematodes, suggesting the two neurons are substantially different than previously thought. The expression of these novel genes was controlled by the previously known regulatory network for subtype differentiation. We also describe unique motif organization within individual gene groups classified by the expression patterns in ASE. Our study paves the way to the complete catalog of the expression profiles of individual C. elegans neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.