ArticleAnalytical chemistry2009
Quantitative measurement of zinc secretion from pancreatic islets with high temporal resolution using droplet-based microfluidics.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- Microfluidics for protein interaction studies: current methods, challenges, and future perspectives.European biophysics journal : EBJ · 2026Review
- Time resolved luminescence of millisecond lifetime dyes in droplet microfluidic systems.Biomicrofluidics · 2026Article
- Dynamic sampling fromLab on a chip · 2024Article
- Unveiling islet heterogeneity using an automated microfluidic imaging system.Scientific reports · 2024Article
- Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets.Cell reports methods · 2023Article
- Automated cellular stimulation with integrated pneumatic valves and fluidic capacitors.The Analyst · 2023Article
- Visualizing hypoxic modulation of beta cell secretions via a sensor augmented oxygen gradient.Microsystems & nanoengineering · 2023Article
- Islet-on-a-chip for the study of pancreatic β-cell function.In vitro models · 2022Review
- In Vitro Disease Models of the Endocrine Pancreas.Biomedicines · 2021Review
- A microfluidic system for monitoring glucagon secretion from human pancreatic islets of Langerhans.Analytical methods : advancing methods and applications · 2021Article
- Active Flow Control and Dynamic Analysis in Droplet Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Review
- Utility of low-cost, miniaturized peristaltic and Venturi pumps in droplet microfluidics.Analytica chimica acta · 2021Article
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- Programmable µChopper Device with On-Chip Droplet Mergers for Continuous Assay Calibration.Micromachines · 2020Article
- Microfluidics for interrogating live intact tissues.Microsystems & nanoengineering · 2020Review
- A microfluidic platform integrating pressure-driven and electroosmotic-driven flow with inline filters for affinity separations.Analytical methods : advancing methods and applications · 2019Article
- Synchronized stimulation and continuous insulin sensing in a microfluidic human Islet on a Chip designed for scalable manufacturing.Lab on a chip · 2019Article
- Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone.Scientific reports · 2019Article
- In Vivo Chemical Monitoring at High Spatiotemporal Resolution Using Microfabricated Sampling Probes and Droplet-Based Microfluidics Coupled to Mass Spectrometry.Analytical chemistry · 2018Article
- Advancement of analytical modes in a multichannel, microfluidic droplet-based sample chopper employing phase-locked detection.Analytical methods : advancing methods and applications · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We assayed glucose-stimulated insulin secretion (GSIS) from live, murine islets of Langerhans in microfluidic devices by the downstream formation of aqueous droplets. Zinc ions, which are cosecreted with insulin from beta-cells, were quantitatively measured from single islets with high temporal resolution using a fluorescent indicator, FluoZin-3. Real-time storage of secretions into droplets (volume of 0.470 +/- 0.009 nL) effectively preserves the temporal chemical information, allowing reconstruction of the secretory time record. The use of passive flow control within the device removes the need for syringe pumps, requiring only a single hand-held syringe. Under stimulatory glucose levels (11 mM), bursts of zinc as high as approximately 800 fg islet(-1) min(-1) were measured. Treatment with diazoxide effectively blocked zinc secretion, as expected. High temporal resolution reveals two major classes of oscillations in secreted zinc, with predominate periods at approximately 20-40 s and approximately 5-10 min. The more rapid oscillation periods match closely with those of intraislet calcium oscillations, while the slower oscillations are consistent with insulin pulses typically measured in bulk islet experiments or in the bloodstream. This droplet sampling technique should be widely applicable to time-resolved cellular secretion measurements, either in real-time or for postprocessing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.