ArticleProtein science : a publication of the Protein Society2010
Enhanced red-emitting railroad worm luciferase for bioassays and bioimaging.
Article in Protein science : a publication of the Protein Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Mapping the structural dynamics of red- and blue-emitting beetle luciferases revealed by HDX-MS.Life science alliance · 2026Article
- Advances in human pluripotent stem cell reporter systems.iScience · 2024Review
- Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications.Biochemistry research international · 2024Review
- A higher spectral range of beetle bioluminescence with infraluciferin.Frontiers in bioengineering and biotechnology · 2022Article
- Bioluminescent Imaging Systems for Assay Developments.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Review
- The elusive relationship between structure and colour emission in beetle luciferases.Nature reviews. Chemistry · 2021Review
- 5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.PloS one · 2020Article
- Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging.Chembiochem : a European journal of chemical biology · 2018Article
- A kidney injury molecule-1 (Kim-1) gene reporter in a mouse artificial chromosome: the responsiveness to cisplatin toxicity in immortalized mouse kidney S3 cells.The journal of gene medicine · 2016Article
- Bioluminescence: a versatile technique for imaging cellular and molecular features.MedChemComm · 2014Article
- Approaches to engineer stability of beetle luciferases.Computational and structural biotechnology journal · 2012Review
- Article
- A general approach for receptor and antibody-targeted detection of native proteins utilizing split-luciferase reassembly.ACS chemical biology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A luciferase from the railroad worm (Phrixothrix hirtus) is the only red-emitting bioluminescent enzyme in nature that is advantageous in multicolor luciferase assays and in bioluminescence imaging (BLI). However, it is not used widely in scientific or industrial applications because of its low activity and stability. By using site-directed mutagenesis, we produced red-emitting mutants with higher activity and better stability. Compared with the wild-type (WT), the luminescent activities from extracts of cultured mammalian cells expressing mutant luciferase were 9.8-fold in I212L/N351K, 8.4-fold in I212L, and 7.8-fold in I212L/S463R; and the cell-based activities were 3.6-fold in I212L/N351K and 3.4-fold in N351K. The remaining activities after incubation at 37 degrees C for 10 min were 50.0% for I212L/S463R, 31.8% for I212L, and 23.0% for I212L/N351K, but only 5.2% for WT. To demonstrate an application of I212L/N351K, cell-based BLI was performed, and the luminescence signal was 3.6-fold higher than in WT. These results indicate that the mutants might improve the practicability of this signaling in bioassays and BLI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.