ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Molecular reconstruction of mGluR5a-mediated endocannabinoid signaling cascade in single rat sympathetic neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- From Classical to Alternative Pathways of 2-Arachidonoylglycerol Synthesis: AlterAGs at the Crossroad of Endocannabinoid and Lysophospholipid Signaling.Molecules (Basel, Switzerland) · 2024Review
- Glutamate Signaling in Hepatic Stellate Cells Drives Alcoholic Steatosis.Cell metabolism · 2019Article
- Homer2 within the central nucleus of the amygdala modulates withdrawal-induced anxiety in a mouse model of binge-drinking.Neuropharmacology · 2018Article
- Glutamate Delta-1 Receptor Regulates Metabotropic Glutamate Receptor 5 Signaling in the Hippocampus.Molecular pharmacology · 2016Article
- Homer protein-metabotropic glutamate receptor binding regulates endocannabinoid signaling and affects hyperexcitability in a mouse model of fragile X syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Biphasic modulation by mGlu5 receptors of TRPV1-mediated intracellular calcium elevation in sensory neurons contributes to heat sensitivity.British journal of pharmacology · 2015Article
- The Endocannabinoid Signaling System in the CNS: A Primer.International review of neurobiology · 2015Review
- Parsing the players: 2-arachidonoylglycerol synthesis and degradation in the CNS.British journal of pharmacology · 2014Review
- Article
- Imbalances in prefrontal cortex CC-Homer1 versus CC-Homer2 expression promote cocaine preference.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The interaction between mGluR1 and the calcium channel Cav₂.₁ preserves coupling in the presence of long Homer proteins.Neuropharmacology · 2013Article
- Potentiation of mGlu5 receptors with the novel enhancer, VU0360172, reduces spontaneous absence seizures in WAG/Rij rats.Neuropharmacology · 2013Article
- N-Arachidonyl glycine does not activate G protein-coupled receptor 18 signaling via canonical pathways.Molecular pharmacology · 2013Article
- The diacylglycerol lipases: structure, regulation and roles in and beyond endocannabinoid signalling.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Dopamine and full-field illumination activate D1 and D2-D5-type receptors in adult rat retinal ganglion cells.The Journal of comparative neurology · 2012Article
- Differential signalling in human cannabinoid CB1 receptors and their splice variants in autaptic hippocampal neurones.British journal of pharmacology · 2012Article
- Uncoupling of the endocannabinoid signalling complex in a mouse model of fragile X syndrome.Nature communications · 2012Article
- Multiple functions of endocannabinoid signaling in the brain.Annual review of neuroscience · 2012Review
- Supply and demand for endocannabinoids.Trends in neurosciences · 2011Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Endocannabinoids (eCB) such as 2-arachidonylglycerol (2-AG) are lipid metabolites that are synthesized in a postsynaptic neurons and act upon CB(1) cannabinoid receptors (CB(1)R) in presynaptic nerve terminals. This retrograde transmission underlies several forms of short and long term synaptic plasticity within the CNS. Here, we constructed a model system based on isolated rat sympathetic neurons, in which an eCB signaling cascade could be studied in a reduced, spatially compact, and genetically malleable system. We constructed a complete eCB production/mobilization pathway by sequential addition of molecular components. Heterologous expression of four components was required for eCB production and detection: metabotropic glutamate receptor 5a (mGluR5a), Homer 2b, diacylglycerol lipase alpha, and CB(1)R. In these neurons, application of l-glutamate produced voltage-dependent modulation of N-type Ca(2+) channels mediated by activation of CB(1)R. Using both molecular dissection and pharmacological agents, we provide evidence that activation of mGluR5a results in rapid enzymatic production of 2-AG followed by activation of CB(1)R. These experiments define the critical elements required to recapitulate retrograde eCB production and signaling in a single peripheral neuron. Moreover, production/mobilization of eCB can be detected on a physiologically relevant time scale using electrophysiological techniques. The system provides a platform for testing candidate molecules underlying facilitation of eCB transport across the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.