ArticleThe Journal of biological chemistry2009
Human ISCA1 interacts with IOP1/NARFL and functions in both cytosolic and mitochondrial iron-sulfur protein biogenesis.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- Nar1 binds the cytosolic iron sulfur cluster assembly targeting complex via a bipartite interaction interface.bioRxiv : the preprint server for biology · 2026Article
- On the evolutionary trail of MagRs.Zoological research · 2024Article
- Mitochondrial targeting sequence of magnetoreceptor MagR: More than just targeting.Zoological research · 2024Article
- Report of the Third Family with Multiple Mitochondrial Dysfunctions Syndrome 5 Caused by the Founder Variant p.(Glu87Lys) inJournal of pediatric genetics · 2018Article
- Atomistic Insights into Cryptochrome Interprotein Interactions.Biophysical journal · 2018Article
- The NMR contribution to protein-protein networking in Fe-S protein maturation.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.The Journal of biological chemistry · 2017Article
- Homozygous p.(Glu87Lys) variant in ISCA1 is associated with a multiple mitochondrial dysfunctions syndrome.Journal of human genetics · 2017Article
- A novel mutation in nuclear prelamin a recognition factor-like causes diffuse pulmonary arteriovenous malformations.Oncotarget · 2017Article
- Deletion of the Proposed Iron Chaperones IscA/SufA Results in Accumulation of a Red Intermediate Cysteine Desulfurase IscS in Escherichia coli.The Journal of biological chemistry · 2015Article
- Copper binding in IscA inhibits iron-sulphur cluster assembly in Escherichia coli.Molecular microbiology · 2014Article
- Mutations in LYRM4, encoding iron-sulfur cluster biogenesis factor ISD11, cause deficiency of multiple respiratory chain complexes.Human molecular genetics · 2013Article
- Protein expression profiles in patients carrying NFU1 mutations. Contribution to the pathophysiology of the disease.Journal of inherited metabolic disease · 2013Article
- The role of mitochondria in cellular iron-sulfur protein biogenesis: mechanisms, connected processes, and diseases.Cold Spring Harbor perspectives in biology · 2013Review
- Iron binding activity is essential for the function of IscA in iron-sulphur cluster biogenesis.Dalton transactions (Cambridge, England : 2003) · 2013Article
- Mammalian iron metabolism and its control by iron regulatory proteins.Biochimica et biophysica acta · 2012Review
- The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation.Molecular biology of the cell · 2012Article
- Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.PloS one · 2012Article
- Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins.The Journal of biological chemistry · 2011Article
- A fatal mitochondrial disease is associated with defective NFU1 function in the maturation of a subset of mitochondrial Fe-S proteins.American journal of human genetics · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Iron-sulfur proteins play an essential role in many biologic processes. Hence, understanding their assembly is an important goal. In Escherichia coli, the protein IscA is a product of the isc (iron-sulfur cluster) operon and functions in the iron-sulfur cluster assembly pathway in this organism. IscA is conserved in evolution, but its function in mammalian cells is not known. Here, we provide evidence for a role for a human homologue of IscA, named IscA1, in iron-sulfur protein biogenesis. We observe that small interfering RNA knockdown of IscA1 in HeLa cells leads to decreased activity of two mitochondrial iron-sulfur enzymes, succinate dehydrogenase and mitochondrial aconitase, as well as a cytosolic iron-sulfur enzyme, cytosolic aconitase. IscA1 is observed both in cytosolic and mitochondrial fractions. We find that IscA1 interacts with IOP1 (iron-only hydrogenase-like protein 1)/NARFL (nuclear prelamin A recognition factor-like), a cytosolic protein that plays a role in the cytosolic iron-sulfur protein assembly pathway. We therefore propose that human IscA1 plays an important role in both mitochondrial and cytosolic iron-sulfur cluster biogenesis, and a notable component of the latter is the interaction between IscA1 and IOP1.
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